4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside CAS:3459-18-5

I-Enzyme Substrate: I-pNAG isetshenziswa kabanzi njengengxenye ethile yama-enzyme ahlukahlukene abandakanyeka ku-hydrolysis yamabhondi e-β-D-glucoside.Lapho la ma-enzyme ehlukanisa i-molecule ye-pNAG, ikhipha i-p-nitrophenol.Lokhu kuvumela abacwaningi ukuthi balinganise futhi balinganise umsebenzi we-enzymatic.

Ama-Enzyme Activity Assays: I-hydrolysis ye-pNAG ngama-enzyme athile ingatholwa futhi ikalwe nge-spectrophotometrically.Lokhu kwenza i-pNAG ilungele ukuhlolwa komsebenzi we-enzyme, lapho inani le-p-nitrophenol elikhiqizwayo lilingana ngokuqondile nomsebenzi we-enzymatic.

Ukuhlolwa Kokudlula Okuphezulu: I-pNAG ivamise ukusetshenziswa ekuhlolweni kokuhlolwa komphumela ophezulu ukuze kuhlonzwe futhi kubonise izici zama-enzyme inhibitors noma ama-activator.Ngokuhlola umthelela wamakhompiyutha ahlukene emsebenzini we-enzymatic, abacwaningi bangakwazi ukuhlonza abantu abangase babe yizidakamizwa noma amamoduli omsebenzi wama-enzyme.

I-Gene Expression Studies: i-pNAG iphinde isetshenziswe ocwaningweni lwebhayoloji yamangqamuzana ukutadisha ukubonakaliswa kwezakhi zofuzo kanye nokulawulwa.Ngokulinganisa umsebenzi we-enzymatic wama-enzyme athile asebenzisa i-pNAG njenge-substrate, abacwaningi bangaphenya umthelela wokuvezwa kofuzo ekusebenzeni kwe-enzyme nomsebenzi.