I-4-Morpholineethanesulfonic acid CAS: 4432-31-9

I-pH Buffering: I-MES inenani le-pKa elizungeze u-6.1, okuyenza ibe isigcinalwazi esisebenzayo kububanzi be-pH obungu-5.5 ukuya ku-6.7.Isiza ukugcina i-pH ezinzile ngokumelana nezinguquko ku-acidity noma i-alkalinity.Lokhu kubaluleke kakhulu ekuhlolweni nasekuhlolweni okudinga indawo ethile ye-pH.

Izifundo ze-Enzyme: I-MES ivame ukusetshenziswa ocwaningweni lwe-enzyme kanye nokuhlola ngenxa yokuhambisana kwayo nama-enzyme ahlukahlukene.Isiza ukugcina izimo ze-pH ezilungile zomsebenzi we-enzyme, iqinisekisa imiphumela enembile nethembekile.

Ukuhlanzwa Kwamaprotheni: I-MES isetshenziswa ezinqubweni zokuhlanza amaprotheni, njenge-chromatography, ukuze kugcinwe ukuzinza nomsebenzi wephrotheni eqondiwe.Isiza ekugcineni ukwakheka kwephrotheni nokusebenza ngesikhathi sezinyathelo zokuhlanza.

I-Electrophoresis: I-MES ivame ukusetshenziswa ezinqubweni ze-gel electrophoresis, ikakhulukazi ukuhlukanisa nokuhlaziya amaprotheni amancane nama-peptide.Umthamo wayo wokubhafa uqinisekisa i-pH ezinzile, ebalulekile ukuze kubonakale ngeso okunembile kanye nokubonakala kwamabhande amaprotheni.

I-Cell Culture: I-MES ivamise ukusetshenziswa ezifundweni ze-cell culture kanye nokwakhiwa kwemidiya njenge-ejenti yokulondoloza.Isiza ukugcina i-pH ingaphakathi kwebanga elifanele lokukhula kweseli, ukusebenza kahle, nezinqubo zamakhemikhali ezinto eziphilayo ngaphandle kokuphazamisa ukusebenza kwamaselula.

Ukusabela Kwamakhemikhali: I-MES ingasetshenziswa futhi njenge-reagent ekuphenduleni kwamakhemikhali njengoba ingasebenza njengesisekelo esibuthakathaka noma i-asidi.Umthamo wayo wokubhafa usiza ukugcina i-pH engashintshi phakathi nokusabela, okuvumela ukulawula okungcono nokukhiqiza kabusha.