I-4-Morpholineethanesulfonic acid CAS:4432-31-9

Ukunciphisa i-pH: I-MES inenani le-pKa elicishe libe ngu-6.1, okwenza kube yi-buffer ephumelelayo ebangeni le-pH eliphakathi kuka-5.5 no-6.7. Isiza ukugcina i-pH ezinzile ngokumelana nezinguquko ku-acidity noma i-alkalinity. Lokhu kuwusizo kakhulu ekuhlolweni nasekuhlolweni okudinga indawo ethile ye-pH.

Izifundo ze-Enzyme: I-MES ivame ukusetshenziswa ocwaningweni lwe-enzyme nasekuhlolweni ngenxa yokuhambisana kwayo nama-enzyme ahlukahlukene. Isiza ukugcina izimo ze-pH ezifanele zomsebenzi we-enzyme, iqinisekisa imiphumela enembile nethembekile.

Ukuhlanzwa Kwamaprotheni: I-MES isetshenziswa ezinqubweni zokuhlanza amaprotheni, njenge-chromatography, ukuze kulondolozwe ukuzinza kanye nomsebenzi weprotheni eqondiwe. Isiza ekugcineni ukwakheka kanye nokusebenza kweprotheni ngesikhathi sezinyathelo zokuhlanza.

I-Electrophoresis: I-MES ivame ukusetshenziswa ezinqubweni ze-gel electrophoresis, ikakhulukazi ekuhlukaniseni nasekuhlaziyeni amaprotheni amancane nama-peptide. Amandla ayo okugcina aqinisekisa i-pH ezinzile, ebalulekile ekubonisweni okunembile nasekuchazeni ama-protein band ngendlela efanele.

Ukukhuliswa Kwamaseli: I-MES ivame ukusetshenziswa ezifundweni zokukhuliswa kwamaseli kanye nokwakheka kwemidiya njenge-ejenti yokuvimba. Isiza ukugcina i-pH ingaphakathi kobubanzi obufanele bokukhula kwamaseli, ukusebenza kahle, kanye nezinqubo ze-biochemical ngaphandle kokuphazamisa imisebenzi yamaseli.

Ukusabela Kwamakhemikhali: I-MES ingasetshenziswa futhi njenge-reagent ekuphenduleni kwamakhemikhali ngoba ingasebenza njengesisekelo esibuthakathaka noma i-asidi. Amandla ayo okugcina amandla asiza ukugcina i-pH engaguquki ngesikhathi sokusabela, okwenza kube lula ukulawula nokuphindaphinda okungcono.