4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4

Umphumela we-4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) uzosebenza njenge-substrate ye-enzyme beta-glucosidase.Le enzyme ihlukanisa isibopho se-glucosidic ye-MUG, okuholela ekukhululweni kwe-4-Methylumbliferon (4-MU) .Ukusetshenziswa kwe-MUG ngokuyinhloko kumkhakha we-microbiology, ikakhulukazi ekutholeni nokuhlonza amagciwane akhiqiza i-beta-glucosidase.I-MUG ivame ukusetshenziswa ekutholweni kwe-Escherichia coli (E. coli) emanzini kanye namasampula okudla.I-E. coli ine-enzyme ye-beta-glucosidase, ekwazi ukwenza i-MUG i-hydrolyze futhi ikhiqize isignali ye-fluorescent phambi kokukhanya kwe-ultraviolet (UV).Uma i-substrate ye-MUG i-hydrolyzed, i-4-MU ekhiqizwayo ikhipha i-fluorescence eluhlaza okwesibhakabhaka, yenza kube lula ukuhlonza amagciwane anomsebenzi we-beta-glucosidase.Le ndlela ivame ukusetshenziswa ekuqaphelweni kwemvelo nasekuhloleni ukuphepha kokudla, njengoba inikeza izindlela ezisheshayo nezibucayi zokuthola ukungcola kwamagciwane.Ngaphezu kokusetshenziswa kwayo ku-microbiology, i-MUG ingasetshenziswa futhi ukutadisha umsebenzi kanye nokuvinjelwa kwe-beta-glucosidase ucwaningo lwe-biochemistry kanye ne-molecular biology.I-fluorescence yayo inika amandla ukulinganiswa kwe-enzyme kinetics futhi ingasetshenziselwa ukuhlola ama-inhibitor okungenzeka noma ama-activator omsebenzi we-beta-glucosidase.Sekukonke, i-MUG iyinhlanganisela eguquguqukayo ethola ukusetshenziswa okubanzi emkhakheni we-microbiology, biochemistry, kanye ne-molecular biology ukuze kutholwe. Umsebenzi we-beta-glucosidase kanye nokuhlonza amagciwane akhiqiza le enzyme.