TOPS CAS:40567-80-4 Intengo Yomkhiqizi

I-Sodium 3-(N-ethyl-3-methylanilino)propanesulfonate, eyaziwa nangokuthi i-MESNa, inomthelela othize kanye nokusebenza ku-biochemistry kanye ne-molecular biology.Ngokuyinhloko isetshenziswa njenge-ejenti yokunciphisa ukuvikelwa kwamaqembu e-thiol (-SH) kumaprotheni nama-peptides.

I-MESNa isebenza njenge-ejenti yokunciphisa enamandla, ekwazi ukuguqula amabhondi e-disulfide (-SS-) kumaprotheni abe ngamaqembu e-sulfhydryl (-SH).Le nqubo yokunciphisa ibalulekile ezinhlelweni ezihlukahlukene zesayensi, okuhlanganisa ukuhlaziywa kwamaprotheni, ukuhlanzwa, kanye nobunjiniyela bamaprotheni.

Ezinye izinhlelo zokusebenza ezithile ze-MESNa zifaka:

I-Protein Denaturation: I-MESNa ingasetshenziselwa ukuguqula amaprotheni ngokuphula izibopho ze-disulfide, ngaleyo ndlela kwembule ukwakheka kwamaprotheni futhi kube lula ukuhlaziya okwengeziwe noma ukuhlanzwa kwamaprotheni.

Ukunciphisa Ukuhlanganiswa Kwamaprotheni: I-MESNa ingavimbela ukuhlanganiswa kwamaprotheni ngokunciphisa amabhondi e-disulfide, iqinisekise ukuzinza kwamaprotheni kanye ne-solubility in solution.

Amalebula Amaprotheni: I-MESNa ingasetshenziselwa ukwethula amalebula noma ukuguqulwa kwezinsalela ezithile ze-cysteine ​​​​kumaprotheni ngokuphula amabhondi e-disulfide nokudalula amaqembu e-sulfhydryl (-SH).

I-Protein Refolding: I-MESNa isetshenziselwa ukusiza ukuphindaphinda kwamaprotheni ngemva kokukhishwa kwe-denaturation ngokunciphisa izibopho ze-disulfide nokusiza ekwakhiweni okulungile kwezakhiwo zamaprotheni.