I-Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), evame ukubizwa ngokuthi i-ABTS, i-chromogenic substrate evame ukusetshenziswa ekuhlolweni kwamakhemikhali ezinto eziphilayo, ikakhulukazi emkhakheni we-enzymology.Kuyinhlanganisela yokwenziwa esetshenziselwa ukukala umsebenzi wama-enzyme ahlukahlukene, kufaka phakathi ama-peroxidase nama-oxidase.
I-ABTS ayinambala ngesimo sayo se-oxidized kodwa iphenduka ibe luhlaza okwesibhakabhaka-luhlaza lapho ifakwa i-enzyme lapho kukhona i-hydrogen peroxide noma umoya-mpilo wamangqamuzana.Lokhu kushintsha kombala kungenxa yokwakheka kwe-cation radical, edonsa ukukhanya ku-spectrum ebonakalayo.
Ukusabela phakathi kwe-ABTS kanye ne-enzyme kukhiqiza umkhiqizo onemibala ongakalwa nge-spectrophotometrically.Ukuqina kombala kuhambisana ngokuqondile nomsebenzi we-enzymatic, okuvumela abacwaningi ukuthi bahlole ngobuningi i-enzyme kinetics, inhibition ye-enzyme, noma ukusebenzisana kwe-enzyme-substrate.
I-ABTS inohlu olubanzi lwezinhlelo zokusebenza emikhakheni eyahlukene, okuhlanganisa ukuxilonga emitholampilo, ucwaningo lwezemithi, nesayensi yokudla.Izwela kakhulu futhi inikeza ububanzi obubanzi obuguquguqukayo, okuyenza ibe ukukhetha okudumile ezivivinyweni eziningi ze-biochemical.