I-NSP-SA-NHS CAS:199293-83-9 Intengo Yomkhiqizi

I-NSP-SA-NHS, eyaziwa nangokuthi i-N-succinimidyl S-acetylthioacetate I-N-hydroxysuccinimide ester, iyinhlanganisela evame ukusetshenziswa njenge-reagent yokuxhumanisa ethize ye-thiol ekuphenduleni kwe-bioconjugation. Umphumela wayo oyinhloko ukwakheka kwezibopho ze-thioester ezizinzile phakathi kwamaqembu e-thiol akhona kuma-biomolecule, njengeprotheni noma ama-peptide.

Ukusetshenziswa kwe-NSP-SA-NHS kugxile kakhulu emkhakheni wokuguqulwa kwamaprotheni kanye nokuvimbela ukunyakaza. Ezinye zezicelo zayo ezibalulekile zifaka:

Ukulebula amaprotheni: I-NSP-SA-NHS isetshenziselwa ukunamathisela amalebula ngokuhlanganyela, njengedayi ezikhanyayo noma i-biotin, kumaprotheni noma ama-peptide. Lokhu kuvumela ukutholwa, ukuhlonza, kanye nokulandelela ama-biomolecule anelebula ezivivinyweni ezahlukene zezinto eziphilayo.

Ukusebenzisana kwamaprotheni: I-NSP-SA-NHS ingasetshenziswa ukuhlanganisa amaprotheni asebenzisanayo ukuze kufundwe ukusebenzisana kwamaprotheni. Lokhu kuvame ukusetshenziswa ezindleleni ezifana nokuhlanganisa i-immunoprecipitation noma ukuhlolwa kokudonsa phansi ukuze kutholakale abalingani ababophayo noma ama-complexes okutadisha amaprotheni.

Ukuvimba amaprotheni: I-NSP-SA-NHS ivumela ukunamatheliswa kwe-covalent kwamaprotheni noma ama-peptide ezindaweni eziqinile, kufaka phakathi ama-agarose beads, ama-magnetic beads, noma ama-microplates. Lokhu kuwusizo ezinhlelweni ezifana nokuhlanzwa kwe-affinity, ukuhlolwa kwezidakamizwa, noma ukuthuthukiswa kwe-biosensor.

Ukuguqulwa kwendawo: I-NSP-SA-NHS ingasetshenziswa ukuguqula izindawo, njengezilayidi zeglasi noma izinhlayiya ezincane, ngamaprotheni noma ama-peptide, okudala izindawo ezisebenza nge-biomolecule zezinhlelo zokusebenza ezahlukahlukene njengokuxilonga, izinhlelo zokulethwa kwemithi, noma amapulatifomu okuzwa nge-bio.