-
2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2
I-2-Nitrophenyl-beta-D-glucopyranoside iyinhlanganisela yamakhemikhali ehlanganisa i-molecule ye-glucopyranoside exhunywe eqenjini le-nitrophenyl.Ngokuvamile isetshenziswa njenge-substrate ekuhlolweni kwe-enzymatic ukuthola kanye nokulinganisa umsebenzi wama-enzyme njenge-beta-glucosidase.Iqembu le-nitrophenyl lingaqhekeka yi-enzyme, okuholela ekukhululweni komkhiqizo onombala ophuzi ongalinganiswa nge-spectrophotometrically.Le nhlanganisela iwusizo ikakhulukazi ekutadisheni i-enzyme kinetics kanye nokuhlolwa okuphezulu kwama-enzyme inhibitors noma ama-activator.Iphinde isetshenziswe ocwaningweni lwamakhemikhali ezinto eziphilayo ukuze kuphenywe nge-carbohydrate metabolism nanjenge-glycosidic-linkage-specific substrate.
-
I-Tris Base CAS:77-86-1 Intengo Yomkhiqizi
I-Tris Base, eyaziwa nangokuthi i-Tromethamine noma i-THAM, iyinhlanganisela yezinto eziphilayo evame ukusetshenziswa emkhakheni we-biochemistry kanye ne-molecular biology.Impushana emhlophe, eyikristalu encibilika kakhulu emanzini futhi inephunga le-amine.I-Tris Base ivamise ukusetshenziswa njenge-ejenti ephazamisayo ukuze kugcinwe i-pH ezinzile ekuhlolweni okuhlukahlukene kwebhayoloji nezinqubo, njengezifundo ze-DNA kanye namaprotheni.Ingasetshenziswa futhi ekwenziweni kwemithi kanye nasekukhiqizweni kwama-ejenti asebenza phezulu.Sekukonke, i-Tris Base iyingxenye ebalulekile ezinhlelweni eziningi zaselabhorethri lapho ukugcinwa kwe-pH enembile kubalulekile.
-
I-4-Morpholineethanesulfonic acid CAS: 4432-31-9
I-4-Morpholineethanesulfonic acid, evame ukwaziwa nge-MES, iyinhlanganisela ye-zwitterionic esebenza njenge-ejenti ephazamisayo ocwaningweni lwebhayoloji nolwe-biochemical.Isiza ukugcina i-pH ezinzile cishe ku-6-7.5 futhi isetshenziswa kabanzi ngenxa yobuthi bayo obuphansi nokuhambisana nezinhlelo ezihlukahlukene zebhayoloji nama-enzyme.I-MES isetshenziswa ku-electrophoresis, izifundo ze-enzyme, isiko lamaseli, ukuhlanzwa kwamaprotheni, nezinye izinqubo zokuhlola ezidinga ukulawulwa kwe-pH okunembayo.
-
4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7
I-4-Nitrophenyl-beta-D-glucopyranoside iyi-substrate evame ukusetshenziswa ekuhloleni kwamakhemikhali ezinto eziphilayo ukuze kuhlolwe umsebenzi wama-enzyme afana ne-β-glucuronidase.Le nhlanganisela i-hydrolyzed yi-enzyme, okuholela ekukhululweni kwe-4-nitrophenol, engalinganiswa ngokusebenzisa i-spectrophotometry.Ukusetshenziswa kwayo kwenza abacwaningi bakwazi ukufunda izici ezihlukahlukene ze-metabolism yezidakamizwa, i-toxicology, kanye nokuxilongwa komtholampilo okuhlobene nokusabela kwe-glucuronidation.
-
I-MOPSO usawoti we-sodium CAS: 79803-73-9
I-MOPSO usawoti we-sodium iyinhlanganisela yamakhemikhali etholakala ku-MOPS (3-(N-morpholino)propanesulfonic acid).Iwusawoti we-zwitterionic buffer, okusho ukuthi iqukethe kokubili ukushaja okulungile nokubi, okuyivumela ukuthi igcine ngempumelelo ukuqina kwe-pH ekuhlolweni okuhlukahlukene kwebhayoloji kanye ne-biochemical.
Ifomu le-sodium likasawoti we-MOPSO linikeza izinzuzo ezifana nokuncibilika okuthuthukisiwe ezixazululweni ezinamanzi, okwenza kube lula ukuphatha nokuzilungiselela.Ivamise ukusetshenziswa njenge-ejenti ephazamisayo kumidiya ye-cell culture, amasu ebhayoloji yamangqamuzana, ukuhlaziya amaprotheni, kanye nokusabela kwe-enzyme.
Usawoti we-sodium we-MOPSO usiza ukugcina i-pH yendawo yokukhula esikweni lamaseli, uhlinzeka ngendawo ezinzile yokukhula nokusebenza kwamaseli.Kumasu ebhayoloji yamangqamuzana, imisa i-pH yezingxube zokusabela kanye namabhafa asebenzayo, iqinisekisa imiphumela enembile nethembekile ku-DNA ne-RNA isolation, i-PCR, ne-gel electrophoresis.
Ibuye isetshenziswe ekuhlaziyeni amaprotheni, isebenza njenge-ejenti ephazamisayo ngesikhathi sokuhlanzwa kwamaprotheni, i-quantification, kanye ne-electrophoresis.Usawoti we-sodium we-MOPSO uqinisekisa izimo ezilungile ze-pH zokuzinza kwamaprotheni kanye nomsebenzi kuzo zonke lezi zinqubo.
-
4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4
I-4-Methylumbelliferyl-beta-D-glucopyranoside iyi-substrate evame ukusetshenziswa ekuhlolweni kwe-enzymatic ukutadisha umsebenzi we-beta-glucosidase enzymes.Uma isetshenziswa yi-beta-glucosidase, ingena ku-hydrolysis, okuholela ekukhishweni kwe-4-methylumbelliferon, engatholwa futhi ibalwe kusetshenziswa i-fluorescence spectroscopy.Le nhlanganisela isetshenziswa kabanzi emkhakheni we-biochemistry, i-molecular biology, kanye ne-biotechnology ekuhloleni umsebenzi wama-enzyme nezinjongo zokuhlola.Impahla yayo ye-fluorescence iyenza ibe bucayi kakhulu futhi ifanele ukusetshenziswa kwe-high-throughput.
-
I-METHYL Beta-D-GLUCOPYRANOSIDE HEMIHYDRATE CAS:7000-27-3
I-Methyl beta-D-glucopyranoside hemihydrate iyinhlanganisela yamakhemikhali eyingxenye ye-glucopyranosides.I-crystalline powder emhlophe encibilika emanzini.Le nhlanganisela ivame ukusetshenziswa njengomthombo wama-carbohydrate kumidiya ye-cell culture kanye ne-substrate yokusabela kwe-enzymatic ocwaningweni lwe-biochemical kanye ne-biotechnological.Ingasebenza njengenhlanganisela yemodeli yokufunda i-carbohydrate metabolism, ezokuthutha, kanye nokusetshenziswa ezinhlelweni ezihlukahlukene zebhayoloji.I-Methyl beta-D-glucopyranoside hemihydrate ithola izinhlelo zokusebenza emkhakheni we-glycobiology, i-enzymology, nokuthuthukiswa kwezidakamizwa, lapho isetshenziswa njengenhlanganisela yethuluzi lokuhlola nokuhlola okuhlukahlukene.
-
I-AMPSO CAS:68399-79-1 Intengo Yomkhiqizi
I-AMPSO, noma i-3-[(1,1-dimethyl-2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid, iyibhafa ye-zwitterionic evamise ukusetshenziswa ocwaningweni lwebhayoloji nolwe-biochemical.Inenani le-pKa elicishe libe ngu-7.9, okuyenza ifanelekele ukugcina izimo ze-pH ezinzile kuzilungiselelo ezihlukahlukene zokuhlola.I-AMPSO ivame ukusetshenziswa kumidiya yamaseli, ukuhlanzwa kwamaprotheni, ukuhlolwa kwama-enzyme, amajeli e-electrophoresis, nokulandelana kwe-DNA.Isiza ukugcina ibanga le-pH elifunekayo, iqinisekise izimo ezifanele zokukhula kwamangqamuzana, ukuzinza kwamaprotheni, umsebenzi wama-enzyme, nokuhlukaniswa okunembile nokuhlaziywa kwama-biomolecules.Ngekhono layo lokumelana nokushintsha kwe-pH okubangelwa ukwengezwa kwama-asidi noma izisekelo, i-AMPSO iyithuluzi elibalulekile ukugcina ukulawulwa kwe-pH okunembayo ochungechungeni lwezivivinyo zebhayoloji nezamakhemikhali ezinto eziphilayo.
-
I-Bis-tris hydrochloride CAS:124763-51-5
I-Bis-tris hydrochloride iyinhlanganisela enezici zokubhafa ezivame ukusetshenziswa ekuhlolweni kwamakhemikhali ezinto eziphilayo kanye nemvelo.Isiza ukugcina i-pH ezinzile futhi isetshenziswa ku-protein electrophoresis, ukuhlolwa komsebenzi wama-enzyme, isiko lamaseli, kanye nokwakheka kwemithi.Umsebenzi wayo oyinhloko ukumelana nezinguquko ku-pH lapho ama-acids noma izisekelo zengezwa esixazululweni, okuyenza ibe ithuluzi elibalulekile ekusetshenzisweni okuhlukahlukene kwesayensi nezimboni.
-
I-Alpha-D-Glucose pentaacetate CAS:3891-59-6
I-Alpha-D-glucose pentaacetate iyinhlanganisela yamakhemikhali etholwe nge-acetylating amaqembu e-hydroxyl e-alpha-D-glucose ngamaqembu amahlanu e-acetyl.Ivamise ukusetshenziswa ku-organic synthesis njengeqembu elivikelayo lamaqembu e-hydroxyl akhona kuma-carbohydrates.Ingasetshenziswa futhi njengenhlanganisela yereferensi ocwaningweni lwamakhemikhali nasekuhlaziyeni, nanjengento yokuqala yokuhlanganiswa kwezinhlanganisela ezahlukahlukene.Ukwengeza, i-glucose pentaacetate iphenywe ngokusetshenziswa kwayo okungaba khona ezinhlelweni zokulethwa kwezidakamizwa, ngenxa yezakhiwo zayo ezilawulwayo zokukhululwa.
-
I-AMPD CAS:115-69-5 Intengo Yomkhiqizi
I-2-Amino-2-methyl-1,3-propanediol, eyaziwa nangokuthi i-AMPD noma i-α-methyl serinol, iyinhlanganisela yamakhemikhali ene-molecular formula C4H11NO2.Utshwala be-amino obuvame ukusetshenziswa njengekhemikhali emaphakathi ekuhlanganiseni kwemithi kanye nezinhlanganisela eziphilayo.I-AMPD yaziwa ngekhono layo lokusebenza njengesilekeleli se-chiral ekuphenduleni kwe-asymmetric, okuyenza ibe yigugu ekukhiqizeni izinhlanganisela ezihlanzekile ze-enantiomerically.Ukwengeza, isetshenziswe njengesithako ekunakekelweni komuntu siqu kanye nemikhiqizo yezimonyo ngezindawo zayo zokuthambisa.
-
I-CAPS SODIUM SALT CAS:105140-23-6
Usawoti we-sodium we-CAPS yisibhafa se-zwitterionic esivame ukusetshenziswa ezinhlelweni zebhayoloji yamangqamuzana ezinto eziphilayo.Inevelu ye-pKa elinganiselwa ku-10.4, okuyenza isebenze kububanzi be-pH obuphakathi kuka-9.7 no-11.1.Usawoti we-sodium we-CAPS usetshenziswa ku-protein electrophoresis, ukusabela kwe-enzymatic, ukuhlolwa kwebhayoloji namakhemikhali, kanye nemidiya yesiko lamaseli.Inikeza ukumelana nezinguquko ze-pH ezibangelwa ukungcola futhi inokuncibilika okuhle emanzini.
