PIPES monosodium usawoti CAS:10010-67-0

I-ejenti yokubhafa: I-HEPES-Na isetshenziswa ngokuyinhloko njenge-ejenti egcina imali ukuze kugcinwe uhla lwe-pH oluzinzile ekuhlolweni kwebhayoloji ne-biochemical.Ingakwazi ukumelana ngempumelelo nezinguquko ze-pH ezibangelwa ukwengezwa kwama-asidi noma izisekelo.

I-Cell Culture: I-HEPES-Na ivamise ukungezwa kumidiya ye-cell culture ukuze kuhlinzekwe ngendawo ye-pH ezinzile nelungile yokukhula kweseli nokusebenza.Kuyasiza ukulwa nokuguquguquka kwe-pH okungenzeka ngenxa yezinqubo ze-metabolic zamangqamuzana aphilayo.

Ama-Enzyme Assays: I-HEPES-Na ivamise ukusetshenziswa njengesivikelo ekuhlolweni kwe-enzyme.Kuyasiza ukugcina i-pH isezingeni eliphezulu lomsebenzi we-enzyme nokuzinza, iqinisekisa imiphumela enembile nethembekile.

Amasu e-Molecular Biology: I-HEPES-Na isetshenziswa kakhulu kumasu ahlukahlukene ebhayoloji yamangqamuzana, njengokuhlukaniswa kwe-DNA ne-RNA, ukukhuliswa kwe-PCR, nokuhlaziywa kwamaprotheni.Isiza ukugcina izimo ze-pH ezinzile phakathi nalezi zinqubo, okubalulekile ekugcineni ubuqotho nokusebenza kwama-molecule ebhayoloji.

I-Electrophoresis: Ku-gel electrophoresis, i-HEPES-Na isetshenziswa njengesivimbeli ukuhlinzeka ngendawo ye-pH ezinzile yokuhlukaniswa kwe-DNA, i-RNA, namaprotheni.Kuyasiza ukuqinisekisa ukufuduka okufanele kanye nokulungiswa kwama-molecule ngaphakathi kwe-matrix yejeli.