Amapayipi usawoti we-monosodium CAS:10010-67-0

I-Agent Yokubhafa: I-HEPES-Na isetshenziswa kakhulu njenge-ejenti yokubopha ukugcina ububanzi be-pH obuzinzile ekuhlolweni kwezinto eziphilayo kanye nezamakhemikhali. Ingamelana ngempumelelo nezinguquko ze-pH ezibangelwa ukwengezwa kwama-acid noma izisekelo.

Ukukhuliswa Kwamaseli: I-HEPES-Na ivame ukungezwa kwimidiya yokukhuliswa kwamaseli ukuze inikeze indawo ye-pH ezinzile nefanele yokukhula kwamaseli kanye nokuphila kahle. Kuyasiza ekulweni nokushintshashintsha kwe-pH okungenzeka ngenxa yezinqubo ze-metabolic zamaseli aphilayo.

Ukuhlolwa Kwe-Enzyme: I-HEPES-Na ivame ukusetshenziswa njenge-buffer ekuhlolweni kwe-enzyme. Isiza ukugcina i-pH isezingeni elihle kakhulu lomsebenzi we-enzyme kanye nokuqina, iqinisekisa imiphumela enembile nethembekile.

Amasu Ebhayoloji Yamamolekyuli: I-HEPES-Na isetshenziswa kabanzi kumasu ahlukahlukene ebhayoloji yamamolekyuli, njengokuhlukaniswa kwe-DNA ne-RNA, ukukhuliswa kwe-PCR, kanye nokuhlaziywa kwamaprotheni. Kuyasiza ekugcineni izimo ze-pH ezizinzile phakathi nalezi zinqubo, okubalulekile ekugcineni ubuqotho nokusebenza kwamamolekyuli ebhayoloji.

I-Electrophoresis: Ku-gel electrophoresis, i-HEPES-Na isetshenziswa njenge-buffer ukuhlinzeka ngendawo ezinzile ye-pH yokuhlukaniswa kwe-DNA, i-RNA, namaprotheni. Kuyasiza ukuqinisekisa ukufuduka okufanele kanye nokuxazululwa kwama-molecule ngaphakathi kwe-gel matrix.