Phenylgalactoside CAS:2818-58-8

Umthelela emsebenzini we-enzyme: I-Phenylgalactoside ivamise ukusetshenziselwa ukukala umsebenzi we-enzyme i-β-galactosidase.Lapho i-phenylgalactoside i-hydrolyzed nge-β-galactosidase, ikhipha i-p-nitrophenol.Ukuqoqwa kwe-p-nitrophenol kungalinganiswa ngobuningi, kunikeze imininingwane ngomsebenzi we-β-galactosidase.Lo mphumela usetshenziswa ezinhlelweni ezifana nezivivinyo zama-enzyme namasistimu okuhlola.

Ukuhlaziywa kwenkulumo yofuzo: I-Phenylgalactoside ivamise ukusetshenziswa njenge-substrate ekuhlolweni kwebhayoloji yamangqamuzana ukuze kufundwe ukuchazwa kofuzo.Ufuzo lwe-lacZ, olufaka i-β-galactosidase, luvamise ukuhlanganiswa nokulandelana kokulawula kwezinye izinhlobo zofuzo ezithakaselwayo.Ukuvezwa kofuzo lwe-lacZ kanye ne-hydrolysis ye-phenylgalactoside nge-β-galactosidase kungabonisa iphethini yenkulumo kanye nezinga lofuzo oluqondiwe olufundwayo.

Amasistimu okuhlola: I-Phenylgalactoside iyingxenye ebalulekile yezinhlelo zokuhlola ezisebenzisa umsebenzi we-β-galactosidase.Isibonelo esisodwa esaziwa kabanzi indlela yokuhlola okuluhlaza okwesibhakabhaka nokumhlophe, esetshenziselwa ukuhlonza amaseli ahlangene noma aguqukile ekuhlolweni kwebhayoloji yamangqamuzana.Amakholoni athathe ngempumelelo i-DNA recombinant noma aphinde ahlangana kabusha nezakhi zofuzo azoveza i-β-galactosidase, okuholela ku-hydrolysis ye-phenylgalactoside kanye nokwakheka kombala oluhlaza okwesibhakabhaka.

Ukuhlanzwa kwamaprotheni: Kwezinye izimo, i-phenylgalactoside ingasetshenziswa njenge-ligand ye-affinity chromatography ukuhlanza amaprotheni abophezela ngokuqondile noma acushwe yi-β-galactosidase.Iphrotheni elithakaselwayo lingase libe nethegi ye-affinity noma umaka wokuhlanganisa oqukethe isizinda esibophezelayo se-β-galactosidase.Ngokudlulisela ingxube yamaprotheni kukholamu ene-phenylgalactoside enganyakazi, iphrotheni oyifunayo ingagcinwa ngokukhetha bese ikhishwa kamuva.