I-ONPG CAS:369-07-3 Intengo Yomkhiqizi

I-O-nitrophenyl-β-D-galactopyranoside (ONPG) iyi-substrate yokwenziwa esetshenziswa ekuhlolweni kwebhayoloji ye-biochemical kanye ne-molecular biology ukukala umsebenzi we-enzyme i-β-galactosidase.Ngokuvamile isetshenziswa ekuhlolweni ukuze kutholwe ukubonakaliswa kofuzo ezinhlelweni zebhaktheriya, njenge-Escherichia coli. I-ONPG iyinhlanganisela engenambala eqhekezwa yi-β-galactosidase, okuholela ekukhishweni kwenhlanganisela ephuzi, i-o-nitrophenol.Umbala ophuzi okhiqizwayo ungakalwa nge-spectrophotometrically, unikeze isilinganiso esingaqondile somsebenzi we-enzyme.Isivivinyo esisetshenziswa i-ONPG sivame ukubizwa ngokuthi i-ONPG assay futhi kuyindlela esetshenziswa kakhulu ocwaningweni lwebhayoloji yamangqamuzana ukuhlola amazinga okuveza izakhi zofuzo ezilawulwa yi-lac. i-operon kumaseli webhaktheriya.

Oda noma Cela I-Quote

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isicelo kanye Nomphumela

Umphumela we-ONPG njenge-substrate uzoqhekeka yi-enzyme i-β-galactosidase, okuholela ekukhishweni komkhiqizo ophuzi, i-o-nitrophenol.Lokhu kushintsha kombala kungalinganiswa nge-spectrophotometrically, okuvumela ukulinganisa komsebenzi we-β-galactosidase.Ukusetshenziswa kwe-ONPG kusekuhlolweni kokuvezwa kofuzo kubhayoloji yamangqamuzana nocwaningo lwe-microbiology.Ngokuvamile isetshenziselwa ukukala umsebenzi we-β-galactosidase njengentatheli yocwaningo lwesisho sezakhi zofuzo, ikakhulukazi kumagciwane afana ne-E. coli.Isakhi sofuzo se-lacZ, esifaka i-β-galactosidase, sivame ukusetshenziswa njengomaka wokuhlaziya isisho sofuzo, njengoba ukusho kwaso kungenziwa izimo ezithile noma kulawulwe abagqugquzeli abathile.Isivivinyo se-ONPG sinikeza indlela elula nethembekile yokuhlola izinga ukubonakaliswa kofuzo ngokulinganisa umsebenzi we-β-galactosidase.Lesi sivivinyo sisetshenziswa kakhulu ezinhlelweni ezahlukahlukene, njengokufunda umsebenzi womgqugquzeli, ukulawulwa kwezakhi zofuzo, nokusebenzisana kwamaprotheni namaprotheni.Ukwengeza, ingasetshenziswa ukunquma i-enzyme kinetics futhi ihlole imiphumela yokuguqulwa noma ukwelashwa emsebenzini we-enzyme.

Isampula Lomkhiqizo

Ukupakishwa komkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	I-C12H15NO8
Isivivinyo	99%
Ukubukeka	Impushana emhlophe
Inombolo ye-CAS.	369-07-3
Ukupakisha	Ezincane nezinkulu
I-Shelf Life	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	ISO.