I-Nitrotetrazolium Blue Chloride CAS: 298-83-9

I-Nitrotetrazolium Blue Chloride (NBT) iyinkomba ye-redox evame ukusetshenziswa ekuhlolweni kwebhayoloji ne-biochemical.Kuyimpushana ephuzi ephaphathekile ephenduka ibe luhlaza okwesibhakabhaka lapho incishisiwe, ikwenze kube usizo ekutholeni ukuba khona kwama-enzyme athile kanye nomsebenzi we-metabolic.

Umphumela oyinhloko we-NBT ukwakheka kwemvula ye-formazan eluhlaza okwesibhakabhaka lapho incishiswa ama-enzyme athile.Lokhu kushintsha kombala kuvumela ukutholwa okubonakalayo noma kwe-spectrophotometric komsebenzi we-enzyme.

I-NBT inezinhlobonhlobo zezinhlelo zokusebenza ocwaningweni nasekuxilongweni.Nazi ezinye zezinsiza zayo eziyinhloko:

Ukuhlolwa komsebenzi wama-enzyme: I-NBT ingasetshenziswa ukukala umsebenzi wama-dehydrogenases, abandakanyeka ezinqubweni ezifana nokuphefumula kwamaselula kanye ne-metabolism.Ngokuqapha ukwehliswa kwe-NBT ku-formazan, abacwaningi bangahlola umsebenzi walawa ma-enzyme.

Ukuhlolwa kokusebenza kwamangqamuzana omzimba omzimba: I-NBT ivame ukusetshenziswa ekuhlolweni kokunciphisa i-NBT ukuhlola umsebenzi wokuqhuma kokuphefumula kwamangqamuzana omzimba, ikakhulukazi ama-phagocyte.Ukuhlolwa kukala ikhono lalawa maseli ukukhiqiza izinhlobo ze-oksijini ezisebenzayo, ezinganciphisa i-NBT futhi zakhe imvula eluhlaza okwesibhakabhaka.

Ucwaningo lwe-Microbiology: I-NBT iqashwe ku-microbiology ukutadisha umzimba we-microbial kanye nokuhlola umsebenzi wama-enzyme athile.Isibonelo, isetshenziselwe ukuthola ama-nitrate reductase noma amagciwane akha i-formazan.

Izifundo zokusebenza kwamaseli: Ukwehliswa kwe-NBT kuvumela abacwaningi ukuthi bahlole umsebenzi we-metabolic nokusebenza kwamaseli.Ngokukala ubukhulu bomkhiqizo we-formazan eluhlaza okwesibhakabhaka, kungenzeka ukunquma inani lamaseli asebenzayo kusampula esinikeziwe.