I-MOPS CAS:1132-61-2 Intengo Yomkhiqizi

Umthelela we-MOPS (3-(N-morpholino)propanesulfonic acid) ngokuyinhloko uhlobene namandla ayo okugcina ibhafa kanye nekhono lokugcina izinga le-pH elizinzile.I-MOPS iyinhlanganisela ye-zwitterionic, okusho ukuthi iqukethe kokubili inkokhiso enhle nebi, eyivumela ukuthi isebenze njengesilondolozi esisebenzayo ezinhlelweni zebhayoloji.

Enye yezinhlelo zokusebenza ezibalulekile ze-MOPS kusikompilo lwamaseli, lapho isetshenziselwa khona ukugcina i-pH yendawo yokukhula.Amaseli adinga i-pH ezinzile ukuze akhule futhi asebenze ngokugcwele, futhi i-MOPS isiza ekugcineni ibhafa okumaphakathi nokuvimbela ukushintshashintsha kwe-pH okungaba yingozi empilweni yeseli.

I-MOPS ijwayele ukusetshenziswa kumasu ebhayoloji yamangqamuzana afana ne-DNA ne-RNA isolation, i-PCR (i-polymerase chain reaction), kanye ne-gel electrophoresis.Kulezi zinhlelo zokusebenza, i-MOPS isiza ukuzinzisa i-pH yezingxube zokusabela kanye namabhafa asebenzayo, iqinisekisa imiphumela enembile nethembekile.

Ekuhlaziyeni amaprotheni, i-MOPS ingasetshenziswa njenge-ejenti ephazamisayo kumasu afana nokuhlanzwa kwamaprotheni, ukulinganisa kwamaprotheni, kanye ne-protein electrophoresis.Kuyasiza ukugcina imvelo efanele ye-pH edingekayo ukuze kuqiniswe amaprotheni kanye nomsebenzi phakathi nalezi zinqubo.

Ukwengeza, i-MOPS ingasetshenziswa ekuphenduleni kwe-enzyme kanye nezifundo ze-enzyme kinetics.Umthamo wayo wokubhafa uvumela ukugcinwa kwezimo ezilungile ze-pH, okubalulekile emsebenzini wama-enzyme kanye nezilinganiso ze-kinetic ezinembile.