I-MOPS CAS: 1132-61-2 Intengo Yomkhiqizi

Umphumela we-MOPS (3-(N-morpholino)propanesulfonic acid) uhlobene kakhulu namandla ayo okugcina izinga le-pH lizinzile. I-MOPS iyinhlanganisela ye-zwitterionic, okusho ukuthi iqukethe kokubili ishaja enhle nembi, eyivumela ukuthi isebenze njenge-buffer ephumelelayo ezinhlelweni zezinto eziphilayo.

Enye yezindlela ezibalulekile zokusebenzisa i-MOPS ekukhuleni kwamaseli, lapho isetshenziswa khona ukugcina i-pH yendawo yokukhula. Amaseli adinga i-pH ezinzile ukuze akhule kahle futhi asebenze kahle, kanti i-MOPS iyasiza ekuvimbeleni indawo yokukhula nokuvimbela ukuguquguquka kwe-pH okungaba yingozi empilweni yamaseli.

I-MOPS isetshenziswa kakhulu nasemasu ebhayoloji yama-molecular njenge-DNA ne-RNA isolation, i-PCR (polymerase chain reaction), kanye ne-gel electrophoresis. Kulezi zinhlelo zokusebenza, i-MOPS isiza ekuqiniseni i-pH yezingxube zokusabela kanye nokusebenzisa ama-buffer, ukuqinisekisa imiphumela enembile nethembekile.

Ekuhlaziyweni kwamaprotheni, i-MOPS ingasetshenziswa njenge-ejenti yokubopha ezindleleni ezifana nokuhlanzwa kwamaprotheni, ukulinganiswa kwamaprotheni, kanye ne-electrophoresis yamaprotheni. Kuyasiza ukugcina indawo efanele ye-pH edingekayo ukuze amaprotheni azinze futhi asebenze phakathi nalezi zinqubo.

Ngaphezu kwalokho, i-MOPS ingasetshenziswa ezifundweni ze-enzyme reactions kanye ne-enzyme kinetics. Amandla ayo okugcina i-buffer avumela ukugcinwa kwezimo ze-pH ezifanele, okubalulekile emsebenzini we-enzyme kanye nokulinganisa okunembile kwe-kinetic.