HEPES-Na CAS:75277-39-3 Intengo Yomkhiqizi

I-buffering agent: I-HEPES kasawoti wesodium ivamise ukusetshenziswa njenge-ejenti egcina isigcina isikhathi ekuhlolweni kwebhayoloji kanye ne-biochemical.Isiza ukugcina ububanzi be-pH obuzinzile, ikakhulukazi ebangeni le-physiological (pH 7.2-7.6).Umthamo wayo wokubhafa uwenza ube wusizo ekugcineni izimo ezifanele zokusabela okuhlukahlukene kwe-enzymatic, isiko lamaseli, nezindlela zebhayoloji yamangqamuzana.

Isiko lamaseli: Usawoti wesodium we-HEPES usetshenziswa kakhulu njenge-ejenti egcina isigcinalwazi kumidiya yamaseli.Amandla okugcina i-pH ezinzile ibalulekile ekukhuleni okufanele nokusebenza kwamaseli.I-HEPES ivamise ukuthandwa kunezinye izisebenzeli zokubhafa njengoba ingabonisi izinguquko eziphawulekayo ku-pH uma ivezwe ku-CO2 yomoya.

Izifundo ze-enzyme: I-HEPES kasawoti wesodium iwusizo ikakhulukazi ezifundweni ze-enzymatic lapho imvelo ye-pH engashintshi futhi elawulwayo idingeka.Ivimbela ukuguquguquka okukhulu kwe-pH ngesikhathi sokuphendula kwe-enzymatic, iqinisekisa umsebenzi ophelele we-enzyme.

I-Electrophoresis: Usawoti we-sodium we-HEPES uvame ukusetshenziswa njenge-ejenti ephazamisayo kumasu ahlukahlukene we-electrophoretic njenge-polyacrylamide gel electrophoresis (PAGE) kanye ne-agarose gel electrophoresis.Isiza ukugcina i-pH yesigcinalwazi, ebalulekile ekuhlukaniseni nasekuhlaziyeni ama-nucleic acid namaprotheni.

Ukuhlolwa kwamakhemikhali ezinto eziphilayo: Usawoti wesodium we-HEPES uvame ukusetshenziswa ekuhlolweni okuhlukahlukene kwamakhemikhali ezinto eziphilayo, okuhlanganisa ukuhlolwa kwama-enzyme, ukuhlolwa komthamo wamaprotheni, nokuhlolwa kwe-spectrophotometric.Isiza ukugcina ibanga le-pH elifunekayo ukuze uthole imiphumela enembile nekwazi ukukhiqizwa kabusha.

Ukwakhiwa kwezidakamizwa: Usawoti we-sodium we-HEPES usetshenziswa futhi ekwenziweni kwemithi njenge-ejenti ephazamisayo ukuze kunzinziswe ukwakheka kwezidakamizwa nokugcina uhla lwe-pH olufunekayo.