I-HEPES-Na CAS:75277-39-3 Intengo Yomkhiqizi

Usawoti we-HEPES sodium, owaziwa nangokuthi usawoti we-N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid sodium, uyi-zwitterionic buffer evame ukusetshenziswa ocwaningweni lwezinto eziphilayo kanye nezamakhemikhali. Umsebenzi wayo oyinhloko ukugcina izinga le-pH elizinzile ezinhlelweni ezahlukene zokuhlola. Usawoti we-HEPES sodium uguquguqukayo kakhulu, uzinzile, futhi awunabo ubuthi, okwenza ufanelekele izinhlelo eziningi ezahlukene okuhlanganisa ukukhuliswa kwamaseli, i-enzyme kinetics, i-immunoassays, kanye nokuhlolwa kwe-molecular biology. Ihambisana namasampula amaningi ezinto eziphilayo kanye nezinhlelo, inikeza indawo elawulwayo yokulawulwa kwe-pH okunembile.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

I-ejenti yokuvimba: Usawoti we-HEPES sodium uvame ukusetshenziswa njenge-ejenti yokuvimba ekuhlolweni kwezinto eziphilayo kanye nezamakhemikhali. Kuyasiza ekugcineni ububanzi be-pH obuzinzile, ikakhulukazi ebangeni lezinto eziphilayo (pH 7.2-7.6). Amandla ayo okuvimba ayenza ibe usizo ekugcineni izimo ezifanele zokusabela okuhlukahlukene kwe-enzyme, isiko lamaseli, kanye namasu e-biology yama-molecular.

Ukukhuliswa kwamaseli: Usawoti we-sodium we-HEPES usetshenziswa kakhulu njenge-ejenti yokuvimba kuma-cell culture media. Ikhono lokugcina i-pH ezinzile libalulekile ekukhuleni okufanele nasekuphileni kwamaseli. I-HEPES ivame ukuthandwa kunezinye izinto zokuvimba ngoba ayibonisi izinguquko ezibalulekile ku-pH uma ivezwa ku-CO2 yomoya.

Izifundo ze-enzyme: Usawoti we-sodium we-HEPES uwusizo kakhulu ezifundweni ze-enzyme lapho kudingeka khona indawo ye-pH engaguquki nelawulwayo. Uvimbela ukuguquguquka okukhulu kwe-pH ngesikhathi sokuphendula kwe-enzyme, okuqinisekisa umsebenzi we-enzyme ofanele.

I-Electrophoresis: Usawoti we-sodium we-HEPES uvame ukusetshenziswa njenge-ejenti yokubopha ezindleleni ezahlukene ze-electrophoretic njenge-polyacrylamide gel electrophoresis (PAGE) kanye ne-agarose gel electrophoresis. Kuyasiza ukugcina i-pH ye-buffer, ebalulekile ekuhlukaniseni nasekuhlaziyeni ama-nucleic acid namaprotheni.

Ukuhlolwa kwamakhemikhali: Usawoti we-sodium we-HEPES uvame ukusetshenziswa ezinhlotsheni ezahlukene zokuhlolwa kwamakhemikhali, okuhlanganisa ukuhlolwa kwama-enzyme, ukuhlolwa kokulinganisa amaprotheni, kanye nokuhlolwa kwe-spectrophotometric. Kusiza ukugcina ububanzi be-pH obufunekayo ukuze kube nemiphumela enembile nephindaphindayo.

Ukwakheka kwemithi: Usawoti we-HEPES sodium usetshenziswa futhi ekwakhiweni kwemithi njenge-ejenti yokubopha ukuze kuzinziswe ukwakheka kwemithi futhi kugcinwe ububanzi be-pH obufunekayo.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C8H19N2NaO4S
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	75277-39-3
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.