FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS:102286-67-9

I-Fluorescein mono-beta-D-galactopyranoside, eyaziwa nangokuthi i-FMG, iyinhlanganisela ye-fluorescent evame ukusetshenziswa njenge-substrate ekuhlolweni okuhlukahlukene kwe-biochemical and cell biology.Itholakala ku-methyl-beta-D-galactopyranoside ngokuyihlanganisa ne-fluorescein molecule.I-FMG isetshenziswa kabanzi ukutadisha umsebenzi we-beta-galactosidase, i-enzyme eyenza i-hydrolysis ye-lactose ibe i-galactose ne-glucose.Ngokusebenzisa i-FMG njenge-substrate, abacwaningi bangaqapha umsebenzi we-enzymatic we-beta-galactosidase ngesilinganiso sokukhishwa kwe-fluorescence.I-hydrolysis ye-FMG nge-beta-galactosidase iholela ekukhululweni kwe-fluorescein, okuholela ekwandeni kwesignali ye-fluorescent engalinganiswa.I-FMG ingasetshenziswa njengophenyo lwamangqamuzana ukuze kufundwe ukuhlangana okubophezelayo kwama-lectins (amaprotheni abophezela ngokuqondile kumacarbohydrates) kuma-carbohydrate aqukethe i-galactose.Ukubophezela kwe-FMG-lectin complexes kungatholwa futhi kulinganiswe ngokusekelwe ezinguqulweni zokukhishwa kwe-fluorescence.Sekukonke, i-FMG iyithuluzi eliguquguqukayo lokutadisha umsebenzi we-enzyme nokuqashelwa kwe-carbohydrate, enikeza indlela elula nezwelayo yokulinganisa i-fluorescence nokuhlola lezi zinqubo zezinto eziphilayo.

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Isicelo kanye Nomphumela

I-Fluorescein mono-beta-D-galactopyranoside (FMG) iyi-molecule evame ukusetshenziswa ocwaningweni lwebhayoloji njengengxenye engaphansi ukuze kutholwe ubukhona nomsebenzi we-beta-galactosidase enzyme.I-FMG iphuma ku-sugar lactose futhi ihlanganiswa ne-molecule ye-fluorescein.

Umphumela oyinhloko we-FMG ukuthi i-hydrolyzed ngokuqondile nge-beta-galactosidase, i-enzyme ephula i-lactose ibe i-galactose ne-glucose.Le hydrolysis enzymatic ye-FMG iholela ekukhululweni kwe-fluorescein, ekhipha isignali eqinile ye-fluorescence.

Ukusetshenziswa okuyinhloko kwe-FMG kusekutholeni nasekulinganiseni umsebenzi we-beta-galactosidase kumasampuli ahlukahlukene.Le enzyme itholakala ezintweni eziningi eziphilayo, okuhlanganisa amabhaktheriya namaseli ezincelisayo, futhi umsebenzi wayo ungaba inkomba yezinqubo ezihlukahlukene zamangqamuzana kanye nezindlela ze-metabolic.

Ngokusebenzisa i-FMG njenge-substrate, umsebenzi we-beta-galactosidase ungalinganiswa ngokuqapha i-fluorescence ekhishwa i-fluorescein ekhululiwe.Lesi silinganiso singenziwa ngokusetha okuhlukahlukene kokuhlola, okuhlanganisa ukuhlolwa kwe-in vitro kanye nezifundo zokuthwebula izithombe zamaseli bukhoma.

Ngaphezu kwalokho, i-FMG ingasetshenziswa njengethuluzi lokufunda ukusatshalaliswa nokwenziwa kwasendaweni kwe-beta-galactosidase ngaphakathi kwamaseli.Ngokusebenzisa amasu e-fluorescent microscopy, abacwaningi bangabona ngeso lengqondo i-fluorescence ekhishwa yi-FMG phezu kwe-hydrolysis, okubavumela ukuthi balandelele umsebenzi wendawo kanye nowesikhashana we-beta-galactosidase.

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Ulwazi Olwengeziwe:

Ukwakheka	I-C26H22O10
Isivivinyo	99%
Ukubukeka	Impushana emhlophe
Inombolo ye-CAS.	102286-67-9
Ukupakisha	Ezincane nezinkulu
I-Shelf Life	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	ISO.