I-DDT CAS:3483-12-3 Intengo Yomkhiqizi

I-DL-Dithiothreitol, eyaziwa nangokuthi i-DTT, iyi-ejenti yokunciphisa evame ukusetshenziswa ocwaningweni lwebhayoloji yamakhemikhali namangqamuzana.Kuyi-molecule encane eneqembu le-thiol (eliqukethe isulfure) ekugcineni ngakunye.

I-DTT ivame ukusetshenziselwa ukunqamula izibopho ze-disulfide kumaprotheni, esiza ukuwavula noma ukuwaguqula.Lokhu kwehliswa kwamabhondi e-disulfide kubalulekile ezinqubweni zaselabhorethri ezihlukahlukene ezifana nokuhlanzwa kwamaprotheni, i-gel electrophoresis, nezifundo zesakhiwo samaprotheni.I-DTT ingasetshenziswa futhi ukuvikela amaqembu e-thiol nokuvimbela i-oxidation phakathi nezinqubo zokuhlola.

I-DTT ivamise ukungezwa ezixazululweni zokuhlola ezindaweni ezincane, futhi umsebenzi wayo uncike ekubeni khona komoyampilo.Kubalulekile ukuphatha i-DTT ngokucophelela njengoba izwela emoyeni, ukushisa, nomswakama, okunganciphisa ukusebenza kwayo.

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Ukwehliswa kwamabhondi e-Disulfide: I-DTT ngokuyinhloko isetshenziselwa ukunqamula izibopho ze-disulfide, okuyizibopho ezihlangene ezakhiwe phakathi kwezinsalela ezimbili ze-cysteine kumaprotheni.Ngokunciphisa lezi zibopho, i-DTT isiza amaprotheni e-denature, okwenza ukutadisha kwesakhiwo nokusebenza kwawo.

Ukugoqa Amaprotheni: I-DTT ingasiza ekugoqeni amaprotheni ngendlela efanele ngokuvimbela ukwakheka okungalungile kwebhondi ye-disulfide.Yehlisa noma yimaphi amabhondi e-disulfide okungewona awomdabu angase akheke ngesikhathi sokugoqa amaprotheni, okuvumela iphrotheni ukuthi isebenzise ukwakheka kwayo komdabu.

Umsebenzi we-Enzyme: I-DTT ingenza ama-enzyme athile asebenze ngokunciphisa noma yimaphi amabhondi e-disulfide avimbelayo akhona.Ukwengeza, i-DTT ingavimbela i-oxidation yezinsalela ze-cysteine ezibalulekile, okungase kudingeke emsebenzini we-enzyme.

Ukukhiqizwa Kwamasosha omzimba: I-DTT ivamise ukungezwa ukuze kuncishiswe amabhondi e-disulfide ngesikhathi kukhiqizwa amasosha omzimba.Kuyasiza ukuvimbela ukwakheka kwamabhondi e-disulfide angalungile, okungase kuvimbele ukubopha okufanelekile kwe-antigen.

Ukuzinzisa Amaprotheni: I-DTT ingasetshenziswa ukuzinzisa amaprotheni ngokuvimbela i-oxidation noma ukuhlanganisa kwawo.Isiza ukugcina isimo esincishisiwe samaprotheni ngesikhathi sokugcina nezinqubo zokuhlola.

Ama-ejenti Okunciphisa Kwi-Molecular Biology: I-DTT ivamise ukusetshenziswa kumasu ahlukahlukene webhayoloji yamangqamuzana njengokulandelana kwe-DNA, i-PCR, nokuhlanzwa kwamaprotheni.Ingasiza ukugcina isimo esincishisiwe sezingxenye ezibucayi, iqinisekise imiphumela yokuhlola elungile.

Isampula Lomkhiqizo

Ukupakishwa komkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	I-C4H10O2S2
Isivivinyo	99%
Ukubukeka	Impushana emhlophe
Inombolo ye-CAS.	3483-12-3
Ukupakisha	Ezincane nezinkulu
I-Shelf Life	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	ISO.