5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium usawoti CAS:129541-41-9

Ukutholwa kwe-GUS: I-X-Gluc ihlukaniswa yi-enzyme ye-GUS ibe isakhiwo esiluhlaza okwesibhakabhaka esinganyibiliki esibizwa ngokuthi i-5-bromo-4-chloro-3-indole (X-Ind).Lokhu kusabela kuvumela ukubonwa kanye nokulinganisa komsebenzi we-GUS kumaseli namathishu.

Izifundo ze-Gene expression: I-X-Gluc isetshenziswa njenge-molecule yentatheli ezifundweni zesisho sofuzo.Ngokuhlanganisa isakhi sofuzo se-GUS kumgqugquzeli wentshisakalo, abacwaningi banganquma umsebenzi kanye nephethini yokubonisa yendawo-yesikhashana yomthuthukisi ngokutholwa komsebenzi we-GUS kusetshenziswa i-X-Gluc.

Ukuhlaziywa kwezitshalo ze-Transgenic: Uhlelo lwe-GUS reporter gene lusetshenziswa kakhulu kubhayoloji yamangqamuzana ezitshalo.I-X-Gluc staining ivumela abacwaningi ukuthi bathole futhi bafunde amaphethini e-transgene expression ezitshalweni.Lokhu kusiza ekuqondeni ukulawulwa kwezakhi zofuzo, ukubonakaliswa okuqondene nezicubu, kanye nesayensi yokuthuthuka kwezitshalo.

Ubunjiniyela bezofuzo: I-X-Gluc isetshenziswa njengomaka okhethwayo ekuhlolweni kobunjiniyela bofuzo.Ngokuxhumanisa isakhi sofuzo se-GUS nofuzo lwangaphandle lwentshisekelo, ukugcotshwa kwe-X-Gluc kungasetshenziswa ukukhomba ukuguqulwa okuyimpumelelo nokuhlanganiswa kwezakhi zofuzo ezifiselekayo entweni ephilayo.

Ucwaningo lwe-Microbiology: I-X-Gluc ingasetshenziswa ukuthola nokuhlonza amagciwane akhiqiza i-GUS.I-enzyme i-GUS itholakala ezinhlotsheni eziningi zamabhaktheriya ahlukene, futhi ukugcotshwa nge-X-Gluc kuvumela ukubonakala nokuhlonza amagciwane ane-GUS-positive ezifundweni ze-microbiological.