5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt CAS:18656-96-7

Umphumela we-5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt (X-glucuronide) yikhono layo lokukhiqiza ushintsho oluhlaza okwesibhakabhaka noma umbala lapho kukhona i-enzyme i-beta-D-glucuronidase. Lokhu kusabela kwe-enzymatic kwenzeka lapho isibopho se-glycosidic phakathi kweqembu le-indole kanye ne-glucuronic acid moiety sihlukaniswa yi-beta-D-glucuronidase.

Ukusetshenziswa kwe-X-glucuronide kugxile kakhulu ekuhlolweni kwe-molecular biology kanye ne-enzymology. Ngokuvamile kusetshenziswa njenge-substrate ukuthola umsebenzi we-beta-D-glucuronidase. Ezinye izinhlelo zokusebenza ezithile zifaka:

Ukuhlolwa kwezakhi zofuzo zentatheli: I-X-glucuronide ivame ukusetshenziswa njengesisekelo ekuhlolweni kwezakhi zofuzo zentatheli lapho i-beta-D-glucuronidase isetshenziswa khona njenge-enzyme yentatheli. Ngokuhlanganisa indawo yokuphromotha noma ukulandelana kwekhodi yezakhi zofuzo ezithakaselwayo ku-beta-D-glucuronidase gene, abacwaningi bangalinganisa ukuvezwa noma umsebenzi wezakhi zofuzo ngokuqapha ukwakheka kwe-blue precipitate noma ushintsho lombala lapho i-X-glucuronide ingezwa.

Ukuhlolwa kokuguqulwa kwamagciwane kanye nemvubelo: I-X-glucuronide ingasetshenziswa ukuthola ukuguqulwa okuphumelelayo kwamagciwane noma imvubelo nge-vector equkethe i-gene ye-beta-D-glucuronidase. Amaseli aguquliwe aveza i-enzyme angakhipha i-X-glucuronide, akhiqize ushintsho lombala olubonakalayo noma ukushesha.

Ukupenda nge-histochemical: I-X-glucuronide isetshenziswa kumasu okupenda nge-histochemical ukubona ngeso lengqondo ukuba khona nokusatshalaliswa komsebenzi we-beta-D-glucuronidase ezicutshini noma emaseli. Lokhu kupenda kuvumela indawo kanye nokuhlonza amaseli aveza i-enzyme.