8-Anilino-1-naphthalenesulfonic acid CAS:82-76-8

I-8-Anilino-1-naphthalenesulfonic acid, eyaziwa nangokuthi i-ANS, iyidayi ekhanyayo evame ukusetshenziswa ocwaningweni lwe-biochemical kanye ne-biophysical. Iyingxenye yesigaba samakhemikhali e-organic aziwa ngokuthi ama-sulfonic asekelwe ku-aniline. I-ANS yaziwa ngekhono layo lokubopha ezindaweni ezihlala emanzini noma ezindaweni ezihlala emanzini ezibonakala zigcwele amanzi kumaprotheni nakwamanye ama-biomolecule.

I-ANS iyakhanya uma iboshelwe ezindaweni ezithinta amanzi, okwenza kube yiprobe ewusizo yokufunda izinguquko zokwakheka kwamaprotheni, ukusebenzisana kwamaprotheni namaphrotheni, kanye nezinqubo zokugoqa/ukuvuleka kwamaprotheni. Ukubopha kwe-ANS ezindaweni ezithinta amanzi kuholela ekushintsheni okuluhlaza okwesibhakabhaka ku-emission spectrum, okungaqashwa kusetshenziswa i-fluorescence spectroscopy.

I-ANS ivame ukusetshenziswa ezinhlobonhlobo zezindlela zocwaningo, okuhlanganisa i-fluorescence polarization, i-fluorescence resonance energy transfer (FRET), kanye ne-thermal stability assays. Isetshenziswe ukutadisha isakhiwo kanye nokuqina kwamaprotheni, ukusebenzisana kwamaprotheni ne-ligand, kanye nezinqubo zokuhlanganisa amaprotheni.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

I-probe ye-Fluorescent: I-ANS isetshenziswa kabanzi njenge-probe ye-fluorescent ukutadisha izinguquko zokwakheka kwamaprotheni, izinqubo zokugoqa/ukuvuleka kwamaprotheni, kanye nokusebenzisana kwamaprotheni ne-ligand. Ibopha ezindaweni ezithinta amanzi noma izindawo ezivezwe ngama-hydrophobic kumaprotheni, okuholela ekukhishweni kwe-fluorescence okungaqashwa kusetshenziswa i-fluorescence spectroscopy.

Ukutholwa kokungabi namandla okusebenzisa amanzi: I-ANS iyazwela ezinguqukweni zokungabi namandla okusebenzisa amanzi endaweni encane. Ingasetshenziswa ukuthola nokulinganisa ama-surfactants, ama-detergent, nezinye izinto ezibangela ukungangeni kwamanzi.

Ukuhlolwa kokuqina kwamaprotheni: I-ANS ivame ukusetshenziswa ekuhlolweni kokuqina kokushisa ukuhlola ukuqina kwamaprotheni. Ngokuqapha ukuqina kwe-fluorescence kwamaprotheni aboshwe yi-ANS ngaphansi kwamazinga okushisa ahlukene, kungenzeka ukunquma izici zokuguquka kokushisa kanye nokushisa okuncibilikayo (Tm) kweprotheni.

Izifundo zokuhlanganiswa kwamaprotheni: I-ANS ingasetshenziswa ukutadisha izinqubo zokuhlanganiswa kwamaprotheni, njengokwakheka kwama-amyloid fibrils. Idayi iyazwela ezindaweni ezingabonakali ze-hydrophobic kuma-protein aggregates, okwenza kube ithuluzi eliwusizo lokuqapha inqubekela phambili kanye ne-kinetics yokuhlanganiswa.

Ukuchaza ukusebenzisana kwamaprotheni: I-ANS isetshenziswe ukuphenya ukusebenzisana kwamaprotheni. Lapho amaprotheni esebenzisana, kungase kube noshintsho ekungangeni kwawo amanzi emzimbeni, okungaqashwa kusetshenziswa i-ANS fluorescence.

Ukutholakala kwemithi kanye nokuthuthukiswa kwayo: I-ANS ingasetshenziswa ekuhlolweni kwemithi engaba khona. Ingasiza ekuhloleni ukuhlangana kokubopha kanye nokusebenzisana kwama-molecule amancane namaprotheni aqondiwe, okuvumela abacwaningi ukuthi bathole ama-compounds angaguqula ukusebenza kweprotheni.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C16H13NO3S
Ukuhlolwa	99%
Ukubukeka	Impuphu eluhlaza okotshani
Inombolo ye-CAS	82-76-8
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.