4-Fluoro-7-nitrobenzofurazan CAS:29270-56-2

I-Fluorescent Probe: I-FNB ivame ukusetshenziswa njenge-fluorescent probe. Isabela nama-amine ayinhloko ukuze yakhe imikhiqizo ekhanyayo kakhulu. Lesi sici sivumela i-FNB ukuthi isetshenziselwe ukulebula nokuthola amaprotheni, ama-peptide, kanye nezinye izinto eziqukethe ama-amino.

Ukuhlaziywa kwe-Amino Acid: I-FNB ibalulekile kumasu okuhlaziya ama-amino acid, ikakhulukazi ekususeni ama-amino acid. Isabela ngokukhetha nama-amine ayinhloko kuma-amino acid, yakha ama-derivatives angahlukaniswa futhi alinganiswe ngamasu e-chromatographic.

I-Enzyme Kinetics: I-FNB isetshenziselwa ukufunda i-enzyme kinetics. Ingasetshenziswa ukukala amazinga okusabela kwama-enzyme akhiqiza noma adla ama-amine ayinhloko. Ushintsho ekujuleni kwe-fluorescence kwe-FNB derivative ngesikhathi se-enzyme reaction lunganikeza ulwazi olubalulekile mayelana nomsebenzi wama-enzyme.

Ukulandelana kwe-Nucleic Acid: I-FNB isetshenziswa ezindleleni zokulandelana kwe-nucleic acid ukuthola nokuhlonza ama-nucleotide athile. Ingasabela ngqo nama-amine ayinhloko kuma-nucleotide, okuvumela ukunqunywa kokulandelana kwe-nucleotide ngesikhathi senqubo yokulandelana.