I-Tris(hydroxymethyl)nitromethane CAS:126-11-4

Umthelela:

Umthamo Wokubhafa: I-Tris isebenza njenge-ejenti esebenzayo yokubhafa ngenxa yekhono layo lokwamukela noma lokunikela ngamaphrothoni, igcina uhla lwe-pH oluzinzile kuzisombululo.Ivamise ukusetshenziswa njengengxenye eyinhloko kumasistimu we-buffer ukuzinzisa i-pH yamasampula ebhayoloji nokusabela.

Izicelo:

Ibhayoloji yamangqamuzana: I-Tris isetshenziswa kabanzi njenge-ejenti ephazamisayo kumasu ahlukahlukene webhayoloji yamangqamuzana, okuhlanganisa i-DNA ne-RNA isolation, i-PCR, i-gel electrophoresis, nokuhlanzwa kwamaprotheni.Kuyasiza ukugcina imvelo ye-pH ezinzile, okuvumela izimo ezifanele zokusabela kwe-enzymatic kanye nokusebenzisana kwamangqamuzana.

I-Cell Culture: I-Tris ivame ukusetshenziswa kumidiya ye-cell culture ukuze kugcinwe ibhalansi ye-pH ne-osmotic engashintshi, isekela ukukhula kwamaseli okunempilo nokusebenza.

I-Protein Chemistry: I-Tris isetshenziswa ekuhlolweni kwamakhemikhali amaprotheni, okufana ne-protein solubilization, ukuhlolwa kokuqina kwamaprotheni, kanye nezifundo zokubopha amaprotheni-ligand.Isiza ukugcina ibanga le-pH elifunekayo, iqinisekise ukugoqa okufanele kwamaprotheni nomsebenzi.

I-Enzymology: I-Tris isetshenziswa ekuhlolweni okuhlukahlukene kwe-enzymatic ukuze kuthuthukiswe izimo ze-pH ezidingekayo emsebenzini we-enzymatic.Iqinisekisa imiphumela enokwethenjelwa futhi ekwazi ukukhiqiza kabusha, ivumela ukukala okunembile kwe-enzyme kinetics kanye nezifundo zokuvimbela.

I-Biochemical Assays: I-Tris isetshenziswa njengengxenye ekuhlolweni kwamakhemikhali amaningi e-biochemical ngenxa yezakhiwo zayo zokubhafa.Igcina i-pH engashintshi phakathi nokuhlolwa kwe-colorimetric, spectrophotometric, kanye ne-enzymatic, ithuthukisa ukunemba nokuthembeka kwemiphumela.