I-Tris(hydroxymethyl)nitromethane CAS:126-11-4

Umphumela:

Amandla Okugcina I-Buffering: I-Tris isebenza njenge-ejenti yokugcina i-buffering ephumelelayo ngenxa yekhono layo lokwamukela noma ukunikela ngama-proton, igcina ububanzi be-pH obuzinzile ezixazululweni. Ivame ukusetshenziswa njengengxenye eyinhloko ezinhlelweni zokulondoloza i-buffer ukuze kuzinziswe i-pH yamasampula ebhayoloji kanye nokusabela.

Izicelo:

I-Molecular Biology: I-Tris isetshenziswa kabanzi njenge-ejenti yokubopha ezindleleni ezahlukene ze-molecular biology, okuhlanganisa ukuhlukaniswa kwe-DNA ne-RNA, i-PCR, i-gel electrophoresis, kanye nokuhlanzwa kwamaprotheni. Isiza ekugcineni indawo ye-pH ezinzile, okuvumela izimo ezifanele zokusabela kwe-enzyme kanye nokusebenzisana kwama-molecule.

Ukukhuliswa Kwamaseli: I-Tris ivame ukusetshenziswa emithonjeni yezakhi zofuzo zamaseli ukugcina i-pH eqhubekayo kanye nebhalansi ye-osmotic, isekela ukukhula kwamaseli anempilo kanye nokuphila kahle.

I-Protein Chemistry: I-Tris isetshenziswa ekuhlolweni kwe-protein chemistry, njengokuncibilikiswa kwamaprotheni, ukuhlolwa kokuqina kwamaprotheni, kanye nezifundo zokubopha amaprotheni ne-ligand. Isiza ukugcina uhla lwe-pH olufunwayo, iqinisekisa ukugoqeka kwamaprotheni nokusebenza okufanele.

I-Enzymology: I-Tris isetshenziswa ekuhlolweni okuhlukahlukene kwe-enzymatic ukuze kuthuthukiswe izimo ze-pH ezidingekayo emsebenzini we-enzymatic. Iqinisekisa imiphumela ethembekile nephindaphindwayo, okwenza kube nokulinganisa okunembile kwe-enzyme kinetics kanye nezifundo zokuvinjelwa.

Ukuhlolwa Kwamakhemikhali: I-Tris isetshenziswa njengengxenye ezivivinyweni eziningi zamakhemikhali ngenxa yezakhiwo zayo zokubopha. Igcina i-pH engaguquki ngesikhathi sokuhlolwa kwe-colorimetric, spectrophotometric, kanye ne-enzymatic, okuthuthukisa ukunemba nokuthembeka kwemiphumela.