I-Tris-HCl CAS:1185-53-1 Intengo Yomkhiqizi

I-Tris-HCl, eyaziwa nangokuthi i-Tris hydrochloride, iyi-buffer yezinto eziphilayo evame ukusetshenziswa ezinhlotsheni ezahlukene. Iyinhlanganisela ye-Tris (tris(hydroxymethyl)aminomethane) kanye ne-hydrochloric acid. Lolu hlelo lwe-buffer lusebenza kahle ekugcineni indawo ye-pH ezinzile, ikakhulukazi ebangeni le-pH 7-9. I-Tris-HCl isetshenziswa kabanzi kumasu e-molecular biology, i-protein biochemistry, i-enzymology, kanye nezinye izinhlelo zokusebenza ze-biochemical. Isiza ukugcina izimo ze-pH ezifanele ezidingekayo kulezi zinqubo, ukuqinisekisa ukuzinza nomsebenzi wamaprotheni, ama-enzyme, nama-nucleic acid. I-Tris-HCl itholakala ngezindlela ezahlukene, njenge-powder noma izixazululo ezihlanganisiwe, okwenza kube lula ukuzilungiselela nokusebenzisa ezindaweni ezahlukene zelebhu.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Umthamo wokufaka i-buffering: I-Tris-HCl inamandla okufaka i-buffering amahle kakhulu ebangeni le-pH elingaba ngu-7-9. Ingamelana nezinguquko ku-pH, okwenza ibe yindawo ekahle yokugcina izimo ezizinzile ezivivinyweni eziningi zezinto eziphilayo.

Ukuzinza kwamaprotheni nama-enzyme: I-Tris-HCl ivame ukusetshenziswa njengengxenye yama-buffers ezixazululo zamaprotheni nama-enzyme. Isiza ukugcina ukuzinza nomsebenzi wamaprotheni nama-enzyme ngokuhlinzeka ngendawo edingekayo ye-pH.

Ucwaningo lwe-Nucleic acid: I-Tris-HCl ivame ukusetshenziswa kumasu e-molecular biology, njengokukhipha i-DNA ne-RNA, i-PCR, i-gel electrophoresis, kanye nokulandelana kwe-DNA. Iqinisekisa izimo ze-pH ezifanele zalezi zindlela, ezibalulekile empumelelweni yazo.

Ukusetshenziswa kokukhulisa amaseli: I-Tris-HCl isetshenziswa kwimidiya yokukhulisa amaseli ukugcina i-pH yendawo yokukhula. Iqinisekisa izimo ezifanele zokukhula kwamaseli kanye nokuphila kahle.

Izifundo zokuzinza: I-Tris-HCl isetshenziswa ezifundweni zokuzinza kwemithi neminye imikhiqizo. Isiza ukugcina ukuzinza kwe-pH yamasampula ngesikhathi sokugcina nokuhlola.

Ukuhlolwa kwe-enzyme: Ama-buffer e-Tris-HCl avame ukusetshenziswa ekuhlolweni kwe-enzyme ukuze kulondolozwe i-pH efiselekayo. Ahlinzeka ngendawo efanele yokusebenzisana kwe-enzyme-substrate kanye nokulinganisa okunembile komsebenzi we-enzyme.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C4H12ClNO3
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	1185-53-1
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.