I-Puromycin dihydrochloride CAS: 58-58-2 Intengo Yomkhiqizi

I-Puromycin dihydrochloride iyi-antibiotic enamandla evame ukusetshenziswa ocwaningweni lwe-molecular biology kanye ne-cell biology. Ivimbela ukwakheka kwamaprotheni ngokubangela ukuqedwa kwamaprotheni ngaphambi kwesikhathi ngesikhathi sokuhumusha. I-Puromycin isebenza ngokulingisa ukuphela okungu-3' kwe-aminoacylated tRNA molecule bese ifakwa kuchungechunge lwe-protein olukhulayo. Lokhu kuholela ekunqunyweni kweprotheni futhi ekugcineni kumise inqubo yokuhumusha.

Ukusetshenziswa okuyinhloko kwe-puromycin dihydrochloride kusekukhetheni nasekuhlukaniseni amaseli ashintshwe izakhi zofuzo noma afakwe i-gene yokumelana ne-puromycin. Lokhu kufezwa ngokungenisa i-gene ethakazelisayo kanye ne-gene yokumelana ne-puromycin emaseli, bese kubafaka ekwelashweni kwe-puromycin. Amaseli kuphela ahlanganise ngempumelelo i-gene yokumelana ne-puromycin ku-genome yawo azosinda futhi ande, okuvumela ukukhethwa nokuhlukaniswa kwamaseli afunwayo.

I-Puromycin dihydrochloride isetshenziswa nasezifundweni ezihilela ukubonakaliswa kwezakhi zofuzo kanye nokwakheka kwamaprotheni. Ngokuvimbela ukwakheka kwamaprotheni, ingasiza abacwaningi ukuthi batadishe ukuguquguquka kokwakheka kwamaprotheni, bathole amaprotheni athile akhiqizwa ngokushesha ngenxa yezisusa ezithile, futhi bahlaziye izindlela zokuhumusha.

Ngaphezu kwalokho, i-puromycin dihydrochloride isetshenziselwa ukukhiqiza imigqa yamaseli ezinzile enezinguquko ezithile zofuzo. Ngokufaka i-gene yokumelana ne-puromycin kanye nokuguqulwa kofuzo okuqondiwe, abacwaningi bangakha imigqa yamaseli eveza ngokuzinzile i-gene ethakazelisayo kanye nokumelana ne-puromycin. Lokhu kuvumela izifundo zesikhathi eside kanye nokucaciswa komsebenzi we-gene.