PIPES CAS:5625-37-6 Intengo Yomkhiqizi

PIPES (piperazine-1,4-bisethanesulfonic acid) iyinhlanganisela ye-zwitterionic buffering esetshenziswa ngokuyinhloko ocwaningweni lwebhayoloji nolwemvelo.Inezici ezimbalwa ezibalulekile nezinhlelo zokusebenza, okuhlanganisa:

I-ejenti yokubhafa ye-pH: I-PIPES iyisigcinalwazi esisebenzayo esiza ukugcina ububanzi be-pH obuzinzile ekuhlolweni okuhlukahlukene kwebhayoloji.Isetshenziswa kakhulu emithonjeni yezokuxhumana yamaseli, ukuhlolwa kwama-enzyme, kanye nasekusetshenzisweni kwebhayoloji yamangqamuzana.

Umthamo ophezulu wokubhafa: I-PIPES inomthamo omuhle wokubhafa ngaphakathi kwebanga le-pH elingu-6.1 ukuya ku-7.5, okuyenza ifaneleke ukugcina izimo ze-pH ezinzile ezinhlelweni eziningi zebhayoloji.

Ukusebenzisana okuncane nama-biomolecules: I-PIPES yaziwa ngokugxambukela kwayo okuncane ezinqubweni zamakhemikhali ezinto eziphilayo kanye nokubophezela okuncane kumaprotheni nama-enzyme, okuyenza ibe ilungele ukugcina ubuqotho nomsebenzi wama-biomolecules.

Ifanele izivivinyo ezincike kuzinga lokushisa: I-PIPES iyakwazi ukugcina izakhiwo zayo zokubhafa phezu kwebanga elibanzi lokushisa, okuhlanganisa kokubili amazinga okushisa emvelo kanye naphezulu.Lokhu kuyenza ifaneleke ukuhlolwa okudinga ukuzinza nokunemba ezimeni ezihlukene zokushisa.

Izinhlelo zokusebenza ze-Electrophoresis: I-PIPES ivamise ukusetshenziswa njengesivimbeli kumasu e-gel electrophoresis, njenge-RNA noma i-DNA agarose gel electrophoresis, ngenxa yokumunca kwayo okuphansi kwe-UV kanye nezakhiwo eziphezulu ze-conductivity.

Ukwakhiwa kwezidakamizwa: I-PIPES iphinde isetshenziswe ekwakhiweni kwemithi njenge-ejenti egcina isiphazamiso, ihlinzeka ngokuzinza kanye nokugcina i-pH elungile ukuze umuthi uphumelele.