I-P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

I-P-Nitrophenyl beta-D-lactopyranoside, eyaziwa nangokuthi i-PNPG, iyinhlanganisela evame ukusetshenziswa ekuhlolweni kwe-enzymatic ukukala umsebenzi we-beta-galactosidase, i-enzyme ebandakanyeka kumetabolism ye-carbohydrate.I-PNPG i-substrate yokwenziwa engaqhekeka yi-beta-galactosidase, okuholela ekukhishweni komkhiqizo onombala ophuzi.Ubukhulu be-substrate hydrolysis bungalinganiswa nge-spectrophotometrically ngokulinganisa ukumunca komkhiqizo ngobude obuthile begagasi.Lokhu kuvumela abacwaningi ukuthi bahlole umsebenzi kanye ne-kinetics ye-beta-galactosidase ezimeni ezihlukahlukene, njengokufunda umsebenzi we-enzyme, ukuhlola ama-enzyme inhibitors noma ama-activators, noma ukuhlola imiphumela yokuguqulwa komsebenzi we-enzyme.

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Ukutholwa komsebenzi we-beta-galactosidase: I-PNPG ivamise ukusetshenziswa ekuhloleni ukukala umsebenzi we-beta-galactosidase, i-enzyme eyenza i-hydrolysis ye-lactose ibe yi-glucose ne-galactose.I-hydrolysis ye-PNPG nge-beta-galactosidase ikhipha i-molecule ye-p-nitrophenol (pNP), engatholwa nge-spectrophotometrically ngenxa yombala wayo ophuzi.

Ukuhlolwa kwama-enzyme inhibitors nama-activators: I-PNPG ingasetshenziswa ekuhlolweni komphumela ophezulu ukuze kuhlonzwe izinhlanganisela ezishintsha umsebenzi we-beta-galactosidase.Ngokulinganisa izinga le-PNPG hydrolysis phambi kwezinhlanganisela ezahlukene zokuhlola, abacwaningi bangakwazi ukuhlonza ama-inhibitors anciphisa umsebenzi we-enzyme noma ama-activator athuthukisa umsebenzi we-enzyme.

Ucwaningo lwe-enzyme kinetics: I-hydrolysis ye-PNPG nge-beta-galactosidase ilandela i-Michaelis-Menten kinetics, ivumela abacwaningi ukuthi banqume imingcele ebalulekile ye-enzyme efana nesivinini esikhulu sokusabela (Vmax) kanye ne-Michaelis constant (Km).Lolu lwazi lusiza ekuqondeni ukuhlangana kwe-enzyme ye-substrate nokusebenza kahle kwe-catalytic.

Izinhlelo zokusebenza zebhayoloji yamangqamuzana: I-Beta-galactosidase, ehlukanisa i-PNPG, ivamise ukusetshenziswa njengofuzo lwentatheli kubhayoloji yamangqamuzana.I-substrate ye-PNPG ivamise ukusetshenziselwa ukuthola kanye nokubona ukuvezwa kofuzo lwentatheli, ihlinzeka ngendlela elula nebucayi yokuhlola ukubonakaliswa kofuzo kumasistimu okuhlola ahlukahlukene.

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Ulwazi Olwengeziwe:

Ukwakheka	I-C18H25NO13
Isivivinyo	99%
Ukubukeka	Impushana emhlophe
Inombolo ye-CAS.	4419-94-7
Ukupakisha	Ezincane nezinkulu
I-Shelf Life	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	ISO.