I-NSP-SA-NHS CAS:199293-83-9 Intengo Yomkhiqizi

I-NSP-SA-NHS, eyaziwa nangokuthi i-N-succinimidyl S-acetylthioacetate N-hydroxysuccinimide ester, iyinhlanganisela evamise ukusetshenziswa njenge-thiol-specific crosslinking reagent ekuphenduleni kwe-bioconjugation.Umphumela wawo oyinhloko ukwakhiwa kwezibopho ezizinzile ze-thioester phakathi kwamaqembu e-thiol akhona kuma-biomolecules, njengamaprotheni noma ama-peptide.

Ukusetshenziswa kwe-NSP-SA-NHS ikakhulukazi kumkhakha wokuguqulwa kwamaprotheni kanye nokunganyakazi.Ezinye zezinhlelo zokusebenza zayo ezibalulekile zifaka:

Amalebula amaprotheni: I-NSP-SA-NHS isetshenziselwa ukunamathisela amalebula ngokuhlanganyela, afana nodayi be-fluorescent noma i-biotin, kumaprotheni noma ama-peptide.Lokhu kuvumela ukutholwa, ukuhlonzwa, nokulandelelwa komkhondo wama-biomolecules anelebuli ekuhlolweni okuhlukahlukene kwebhayoloji.

Ukusebenzisana kwamaprotheni-amaprotheni: I-NSP-SA-NHS ingasetshenziselwa ukuwela amaprotheni asebenzisanayo ukuze kufundwe ukusebenzisana kwamaprotheni-amaprotheni.Lokhu kuvame ukusetshenziswa kumasu afana ne-co-immunoprecipitation noma ukuhlola okudonsela phansi ukuze kukhonjwe ozakwethu ababophayo noma ama-protein complexes ocwaningo.

Amaprotheni immobilization: I-NSP-SA-NHS ivumela ukunamathiselwa okuhlangene kwamaprotheni noma ama-peptide endaweni eqinile, okuhlanganisa ubuhlalu be-agarose, ubuhlalu obuzibuthe, noma ama-microplates.Lokhu kuwusizo ezinhlelweni ezifana nokuhlanzwa kobudlelwane, ukuhlolwa kwezidakamizwa, noma ukuthuthukiswa kwe-biosensor.

Ukulungiswa kobuso: I-NSP-SA-NHS ingasetshenziselwa ukuguqula izindawo ezingaphezulu, njengezilayidi zengilazi noma ama-nanoparticles, anamaprotheni noma ama-peptide, ukudala izindawo ezisebenza nge-biomolecule yezinhlelo zokusebenza ezihlukahlukene ezifana nokuxilonga, amasistimu okulethwa kwezidakamizwa, noma izinkundla ze-bio-sensive.