I-Nitro Blue Tetrazolium Chloride monohydrate CAS:298-96-4

Ukusabela kwe-Redox: I-NBT chloride monohydrate idlula ku-redox reaction uma incishiswa ama-enzyme athile noma amanye ama-reducing agents. Lokhu kusabela kuphumela ekwakhekeni kwe-formazan precipitate eluhlaza okwesibhakabhaka, okubonisa ukuba khona kanye nomsebenzi walezi enzymes ze-reductase.

Ukutholwa kwama-enzyme: I-NBT chloride monohydrate isetshenziswa kabanzi ekuhlolweni kwama-enzyme ukuthola umsebenzi wama-enzyme ahlukahlukene e-reductase. Isibonelo, isetshenziselwa ukuthola ama-dehydrogenase, i-succinate dehydrogenase, i-nitroreductase, namanye ama-enzyme e-oxidoreductase. I-blue formazan precipitate eyakhiwe ikhombisa ukuba khona kanye nomsebenzi wala ma-enzyme, okwenza kube wusizo ekuchazeni ama-enzyme kanye nasezifundweni zendawo.

Ukufaka umbala we-histochemical: I-NBT chloride monohydrate isetshenziswa kumasu okufaka umbala we-histochemical ukuze kubonwe ukusatshalaliswa kanye nomsebenzi wama-enzyme ngaphakathi kwezicubu. Ingasetshenziswa ukutadisha indawo yama-enzyme ezinhlotsheni ezithile zamaseli noma ezingxenyeni zezicubu, okuvumela abacwaningi ukuqonda ukubonakaliswa kwama-enzyme kanye nokusebenza ngaphakathi kwamasampula ebhayoloji.

Ukuhlolwa kokuphila kweseli: I-NBT chloride monohydrate isetshenziswa ekuhlolweni kokuphila kweseli ukuhlola umsebenzi we-metabolic kanye nesimo se-redox samaseli. Ukunciphisa i-NBT chloride kubonisa ukuba khona kwama-enzyme asebenzayo e-reductase kumaseli, okuphakamisa ukusebenza kwawo jikelele kanye nomsebenzi we-metabolic.

Izifundo ze-microbiological: I-NBT chloride monohydrate isetshenziswa ezifundweni ze-microbiological ukuthola ukuthi kukhona ama-enzyme athile amagciwane. Le nhlanganisela ingasetshenziswa ukuhlola umsebenzi wama-enzyme athile akhiqizwa amabhaktheriya noma isikhunta, okusiza ekuboneni nasekuchazeni izinhlobo zamagciwane.