I-Iodonitrotetrazolium chloride CAS:146-68-9

Ukutholwa komsebenzi we-dehydrogenase: I-INT ingasetshenziswa ukunquma umsebenzi wama-enzyme e-dehydrogenase kumasampula ebhayoloji. Ama-Dehydrogenase angama-enzyme ahilelekile ekuphefumuleni kwamaseli kanye nezinqubo ezahlukahlukene ze-metabolic. I-INT incishiswa ama-enzyme e-dehydrogenase, okuholela ekwakhekeni komkhiqizo obomvu we-formazan. Ukuqina kombala obomvu kuhambelana ngqo nomsebenzi wama-enzyme e-dehydrogenase akhona kusampula.

Ukuhlolwa kokuphila kweseli: I-INT ingasetshenziswa ukuhlola ukuphila kweseli kanye nomsebenzi we-metabolic. Ngokulinganisa ukwehla kwe-INT ibe yi-formazan, abacwaningi banganquma inani lamaseli asebenzayo kusampula. I-INT encishisiwe yakha i-formazan kuphela kumaseli asebenzayo ngokwe-metabolic, okwenza kube udayi owusizo wokuhlola ukuphila kweseli ezicelweni ezifana nokuhlolwa kobuthi bezidakamizwa noma ukuhlolwa kokukhuliswa kweseli.

Ucwaningo lwe-microbiological: I-INT ivame ukusetshenziswa ku-microbiology ukuthola nokubala ama-microorganism. Ingasetshenziswa ukukhombisa ukukhula kwamagciwane noma isikhunta ngokusekelwe emisebenzini yawo ye-metabolic. Ukuba khona komkhiqizo we-formazan kubonisa ukukhula kwama-microorganisms kusampula.

Ukuxilongwa kwezokwelapha: Ukuhlolwa okusekelwe ku-INT kuthole ukusetshenziswa ekuhlolweni kwezokwelapha. Isibonelo, udayi we-INT ungasetshenziswa ukuhlola umsebenzi wama-neutrophil (uhlobo lweseli elimhlophe legazi) ngokulinganisa ikhono lawo lokunciphisa udayi. Ukungasebenzi kahle kwama-neutrophil kuhlotshaniswa nezifo ezahlukahlukene, kanti ukuhlolwa kwe-INT kunikeza indlela esheshayo nethembekile yokuhlola umsebenzi wawo.