I-Iodonitrotetrazolium chloride CAS:146-68-9 Intengo Yomkhiqizi

I-Iodonitrotetrazolium chloride (INT) ivame ukusetshenziswa ukuthola umsebenzi wama-enzyme e-dehydrogenase ezinhlelweni zezinto eziphilayo. Lawa ma-enzyme ahilelekile ekusetshenzisweni kwe-metabolism yamaseli futhi adlala indima ebalulekile ezindleleni ezahlukahlukene ze-biochemical. I-INT iwusizo kakhulu ekuhlolweni okwenzelwe ukuhlola ukusebenza kweseli kanye nomsebenzi we-metabolism. Uma i-INT ingezwa kusampula yokuhlola, ukunciphisa udayi ngama-enzyme e-dehydrogenase kuphumela ekwakhekeni komkhiqizo obomvu we-formazan. Ukuqina kombala obomvu kungalinganiswa nge-spectrophotometric, kunikeze isilinganiso sobuningi bomsebenzi we-enzyme kanye nokuphila kweseli. Lokhu kwenza i-INT ibe yigugu ezinhlobonhlobo zezicelo, kufaka phakathi ukuhlolwa kokwanda kwamaseli, ukuhlolwa kokuphila kwama-microbial, kanye nokuhlolwa kwemiphumela yemithi ekusetshenzisweni kwe-metabolism yamaseli. Ukusetshenziswa kwayo ocwaningweni nasekuhlolweni kokuxilonga kuye kwaba negalelo ekuqondeni kangcono izinqubo zomzimba neze-pathological ezingeni lamaseli.