I-HEPES CAS: 7365-45-9

I-HEPES idlala indima ebalulekile ekugcineni indawo ye-pH ezinzile ezinhlelweni zokukhulisa amaseli, iqinisekisa ukusebenza nokukhula kwamaseli ngokuvimbela ukuguquguquka kwamazinga e-pH. Leli khono lokuvimba le-HEPES libaluleke kakhulu ekugcineni izimo zomzimba zocwaningo olusekelwe kumaseli nokuhlolwa kwezidakamizwa, okuholela emiphumeleni ethembekile nephindaphindekayo. Ku-biology yama-molecule kanye ne-biochemistry, i-HEPES ivame ukusetshenziswa ekuhlolweni kwama-enzyme ukulawula ukusabela kwe-enzyme okuzwela i-pH ngempumelelo. Amandla ayo okugcina i-pH engaguquki ngaphakathi kobubanzi obuncane (ngokuvamile i-7.2-7.6) abalulekile ekuqinisekiseni ukunemba nokusebenza kahle kwezinqubo ze-biochemical, njengokuhlanzwa kwamaprotheni, ukuhlaziywa kwe-nucleic acid, kanye nezifundo ze-metabolic. I-HEPES isetshenziswa futhi ekusetshenzisweni kwe-electrophoresis, kufaka phakathi i-agarose gel electrophoresis kanye ne-polyacrylamide gel electrophoresis, lapho isiza khona ukudala indawo efanele yokuhlukaniswa kwe-DNA kanye namaprotheni. Izakhiwo zayo zokuvimba zinegalelo kuma-band abukhali futhi achazwe kahle, okuthuthukisa ukuxazululwa nokunemba kwemiphumela yokuhlola. Ngaphezu kwalokho, i-HEPES isetshenziswa kakhulu embonini yezemithi ekwakheni imikhiqizo yezinto eziphilayo, imijovo, kanye nama-reagent okuxilonga. Ukuhambisana kwayo nama-biomolecule azwelayo kanye nobuthi obuphansi kuyenza ibe yisinqumo esifanele sokugcina ukuzinza nokusebenza kwemithi ye-biopharmaceuticals ngesikhathi sokukhiqizwa nokugcinwa kwayo. Sekukonke, i-HEPES isebenza njenge-ejenti yokubopha eguquguqukayo futhi ebaluleke kakhulu enezinhlelo zokusebenza ezahlukahlukene ku-biology yamaseli, ucwaningo lwama-molecular, i-biochemistry, kanye nokuthuthukiswa kwemithi, idlala indima ebalulekile ekuqinisekiseni impumelelo kanye nokuthembeka kokuhlolwa okubanzi kwezinto eziphilayo kanye nezinto eziphilayo.