I-Fmoc-D-Cys(Trt)-OH CAS:167015-11-4

I-Fmoc-D-Cys(Trt)-OH isebenza njengethuluzi eliyisisekelo ekuhlanganisweni kwe-peptide, ikakhulukazi ekwakheni ama-peptide aqukethe i-D-enantiomer ye-cysteine ​​​​ngokunemba. Iqembu elivikela i-Fmoc livikela iqembu lama-amino, liqinisekisa ukusabela okukhethayo ngesikhathi sokuhlanganiswa kwe-peptide, kuyilapho iqembu le-trityl lengeza ungqimba olwengeziwe lokuvikela eqenjini le-thiol. Abacwaningi basebenzisa kakhulu i-Fmoc-D-Cys(Trt)-OH ekwakhiweni kwama-peptide ezinhlelo zokusebenza ezahlukahlukene, okuhlanganisa ukuthuthukiswa kwezokwelapha okusekelwe ku-peptide, izinto eziphilayo, kanye nama-peptide anezimpawu ezithile zesakhiwo. Ukuhambisana kwayo nezindlela ezijwayelekile zokwenziwa kwe-peptide, kokubili kumasu esigaba esiqinile kanye nesigaba sesisombululo, kuthuthukisa ukuguquguquka kwayo emizamweni yocwaningo ehlukahlukene. Ukususwa okulawulwayo kwamaqembu avikelayo kuvumela ukufakwa kwe-D-cysteine ​​​​ngokwesu ku-peptide sequences, kwandisa ububanzi bama-peptide angenziwa. Ngaphezu kwalokho, i-Fmoc-D-Cys(Trt)-OH ithola usizo ezifundweni ezihlobene nobunjiniyela bamaprotheni, ukwakheka kwe-disulfide bond, kanye nokusabela kwe-peptide conjugation. Ukusetshenziswa okunesu kweqembu lokuvikela i-trityl kwenza kube lula ukuphathwa ngendlela enembile kwe-D-cysteine ​​reactivity, okunikeza abacwaningi ithuluzi eliwusizo lokuklama ama-peptide anezinguquko ezenziwe ngokwezifiso. Ngamafuphi, i-Fmoc-D-Cys(Trt)-OH imele njengethuluzi eliguquguqukayo nelibalulekile kumakhemikhali e-peptide, inika abacwaningi amandla okuhlola nokuguqula i-D-cysteine ​​reactivity yezinhlelo zokusebenza eziningi zesayensi, ikakhulukazi ukugxila kuma-peptide aqukethe le amino acid ebalulekile ekucushweni kwe-D.