I-Fmoc-Cys(Kodwa)-OH CAS:67436-13-9

I-Fmoc-Cys(But)-OH isebenza njengethuluzi eliyisisekelo ekuhlanganisweni kwe-peptide, ikakhulukazi ekwakheni ama-peptide aqukethe i-cysteine ​​​​ngokunemba. Iqembu elivikela i-Fmoc livikela iqembu lama-amino, liqinisekisa ukusabela okukhethayo ngesikhathi sokuhlanganiswa kwe-peptide, kuyilapho iqembu le-butyl lengeza ungqimba olwengeziwe lokuvikela eqenjini le-thiol. Abacwaningi basebenzisa kakhulu i-Fmoc-Cys(But)-OH ekwakhiweni kwama-peptide ngezinhlelo ezahlukahlukene, okuhlanganisa ukuthuthukiswa kwemithi esekelwe ku-peptide, i-bioconjugation, kanye nokudalwa kwama-peptide anezici ezithile zesakhiwo. Ukuhambisana kwayo nezindlela ezijwayelekile zokwenziwa kwe-peptide, kokubili kumasu esigaba esiqinile kanye nesigaba sesisombululo, kuthuthukisa ukuguquguquka kwayo emizamweni yocwaningo eyahlukahlukene. Ukususwa okulawulwayo kwamaqembu avikelayo kuvumela ukufakwa kwe-cysteine ​​​​ngokwesu ku-peptide sequences, kwandise ububanzi bama-peptide angenziwa. Ngaphezu kwalokho, i-Fmoc-Cys(But)-OH ithola usizo ekufundweni kokugoqwa kwamaprotheni, ukwakheka kwesibopho se-disulfide, kanye nokuthuthukiswa kwezinto ezisekelwe ku-peptide. Ukusetshenziswa okunesu kweqembu lokuvikela i-butyl kwenza kube lula ukuphathwa ngendlela enembile kwe-cysteine ​​reactivity, okunikeza abacwaningi ithuluzi eliwusizo lokuklama ama-peptide anezinguquko ezenziwe ngokwezifiso. Ngamafuphi, i-Fmoc-Cys(But)-OH imele njengethuluzi eliguquguqukayo nelibalulekile kumakhemikhali e-peptide, inika abacwaningi amandla okuhlola nokuguqula i-cysteine ​​reactivity yezinhlelo zokusebenza eziningi zesayensi, ikakhulukazi ukugxila kuma-peptide aqukethe le amino acid ebalulekile.