I-Fmoc-Asp(OBut)-OSu CAS:78553-23-8

I-Fmoc-Asp(OBut)-OSu isebenza njengethuluzi eliguquguqukayo ekuhlanganisweni kwe-peptide, ikakhulukazi ekwakheni ama-peptide aqukethe i-aspartic acid ngokunemba. Iqembu elivikela i-Fmoc livikela iqembu lama-amino, liqinisekisa ukusabela okukhethayo ngesikhathi sokuhlanganiswa kwe-peptide, kuyilapho iqembu le-O-butyl lengeza ungqimba olwengeziwe lokuvikela eqenjini le-carboxyl. Ukusebenza kwe-OSu ester kusiza ukwakheka kwe-amide bond okuphumelelayo nokukhethayo ngesikhathi sokusabela kokuhlanganiswa kwe-peptide. Abacwaningi basebenzisa kakhulu i-Fmoc-Asp(OBut)-OSu ekwakhiweni kwama-peptide ezinhlelo zokusebenza ezahlukahlukene, okuhlanganisa ukuthuthukiswa kwemithi, izinto eziphilayo, kanye nokuklama ama-peptide asebenzayo anezici ezithile zesakhiwo. Ukuhambisana kwayo nezindlela ezijwayelekile zokwenziwa kwe-peptide, kokubili kumasu esigaba esiqinile kanye nesigaba sesisombululo, kuthuthukisa ukuguquguquka kwayo emizamweni yocwaningo eyahlukahlukene. Ukususwa okulawulwayo kwamaqembu avikelayo kuvumela ukufakwa kwe-aspartic acid ngamasu okulandelana kwe-peptide, kwandise ububanzi bama-peptide angenziwa. Ngaphezu kwalokho, i-Fmoc-Asp(OBut)-OSu ithola usizo ekwakhiweni kwama-peptide ocwaningo lwe-proteomics kanye nokufunda izinqubo zamaseli. Ukusetshenziswa kwesu kweqembu lokuvikela i-O-butyl kwenza kube lula ukuphathwa ngendlela enembile kwe-aspartic acid reactivity, kanti iqembu le-OSu ester lenza kube lula ukuhlanganiswa kwe-bio kanye nokulebula ama-peptide. Ngamafuphi, i-Fmoc-Asp(OBut)-OSu imele njengethuluzi eliyisisekelo nelibalulekile kumakhemikhali e-peptide, inika amandla abacwaningi ukuthi bahlole futhi bashintshe i-aspartic acid reactivity yezinhlelo zokusebenza zesayensi ezahlukahlukene, ikakhulukazi ukugxila kuma-peptide anokusebenza okuthuthukisiwe ngeqembu le-OSu ester.