I-Fmoc-Asn(Trt)-OH CAS:132388-59-1

I-Fmoc-Asn(Trt)-OH ibambe isikhundla esiyinhloko ekwakhiweni kwe-peptide, ikakhulukazi ekuhlanganisweni okulawulwayo kwama-peptide aqukethe i-asparagine. Iqembu elivikela i-Fmoc livikela iqembu lama-amino, liqinisekisa ukusabela okukhethayo ngesikhathi sokuhlanganiswa kwe-peptide, kuyilapho iqembu le-trityl linikeza ungqimba olwengeziwe lokuvikela. Abacwaningi basebenzisa kakhulu i-Fmoc-Asn(Trt)-OH ekwakhiweni kwama-peptide ngezinhlelo ezahlukahlukene, okuhlanganisa ukuthuthukiswa kwemithi, izinto eziphilayo, kanye nokwakhiwa kwama-peptide asebenzayo. Ukuhambisana kwayo nezindlela ezijwayelekile zokwenziwa kwe-peptide, kokubili kumasu esigaba esiqinile kanye nesigaba sesisombululo, kuthuthukisa ukusebenza kwayo emizamweni ehlukahlukene yocwaningo. Ukususwa okulawulwayo kwamaqembu avikelayo kuvumela ukufakwa kwe-asparagine ngamasu okulandelana kwama-peptide, okufaka isandla ekwahlukeni kwama-peptide angakhiwa. Ngaphezu kwalokho, i-Fmoc-Asn(Trt)-OH ithola usizo ekwakhiweni kwama-peptide ocwaningo lwe-proteomics kanye nokutadisha izinqubo zamaseli. Ukusetshenziswa okunesu kweqembu lokuvikela i-trityl kwenza kube lula ukuphathwa ngendlela enembile kwe-asparagine reactivity, okunikeza abacwaningi ithuluzi eliwusizo lokuklama ama-peptide anezinguquko ezithile. Ngamafuphi, i-Fmoc-Asn(Trt)-OH imele njengethuluzi eliguquguqukayo nelibalulekile kumakhemikhali e-peptide, okunika abacwaningi amandla okuhlola nokuguqula i-asparagine reactivity yezinhlelo zokusebenza eziningi zesayensi.