I-DNase I, evela ku-Bovine Pancreas CAS:9003-98-9

I-DNase I, etholakala ku-pancreas yezinkomo, iyi-enzyme ehlukanisa ngqo ama-molecule e-DNA ngokufaka i-hydrolyzing izibopho ze-phosphodiester emgogodleni we-DNA. Umphumela oyinhloko we-DNase I ukuwohloka nokuqhekeka kwe-DNA.

Ukusetshenziswa okuyinhloko kwe-DNase I kusekucwaningweni kwebhayoloji yama-molecule. Ivame ukusetshenziswa ezivivinyweni ezahlukahlukene zokuhlukanisa nokuhlanza i-DNA, ukususa ukungcoliswa kwe-DNA ye-genomic kumasampula e-RNA, nokulawula i-DNA. I-DNase I isebenza kahle kakhulu ekonakaleni i-DNA kuma-complexes e-protein-DNA, okuvumela abacwaningi ukuthi bafunde ukusebenzisana kwe-protein-DNA noma basuse amaprotheni aboshwe yi-DNA.

Ngaphezu kwalokho, i-DNase I ivame ukusetshenziswa ekuhlolweni kwe-DNA footprinting, okusiza ukuhlonza izindawo ze-DNA eziboshwe ngamaprotheni athile. Ngokwelapha isampula ye-DNA nge-DNase I bese kuhlaziywa izingcezu ze-DNA eziphumayo, abacwaningi banganquma izindawo zokubopha zamaprotheni abophezela i-DNA.

I-DNase I isetshenziswa nasekuhlaziyweni kwe-chromatin, lapho isiza ekugayweni kwe-chromatin (i-DNA namaprotheni ahlobene nayo) ukuze ifunde isakhiwo sayo kanye nokuhleleka kwayo. Ngokugaya i-chromatin nge-DNase I, abacwaningi bangabona izindawo ezifinyeleleka kalula futhi ezizwela kakhulu ekuqhekekeni, okuhlinzeka ngemininingwane ngokulawulwa kwezakhi zofuzo kanye nesakhiwo se-chromatin.

Ngaphezu kwalokho, i-DNase I inezinhlelo zokusebenza ekuxilongweni kwezokwelapha, ikakhulukazi ekutholeni nasekulinganiseni i-DNA engenamaseli egazini elijikelezayo. Ngokusebenzisa i-DNase I ukugaya i-DNA engajikelezi, kungenzeka ukuhlaziya nokuthola ukulandelana kwe-DNA ethile ehlotshaniswa nezifo noma ukukhubazeka kwezakhi zofuzo.