I-DAPI (4,6-Diamidini-2-phenylindole dihydrochloride) I-CAS:28718-90-3

I-DAPI (4,6-Diamidino-2-phenylindole dihydrochloride) iyidayi ekhanyayo evame ukusetshenziswa ocwaningweni lwezinto eziphilayo ukuze kubonwe i-DNA. Ibopha emseleni omncane we-DNA enezintambo ezimbili futhi ikhiphe ukukhanya okuluhlaza okwesibhakabhaka uma ikhanyiswa ukukhanya kwe-ultraviolet. I-DAPI ivame ukusetshenziswa ku-microscopy ye-fluorescence ukuze ifake amabala kuma-nuclei eseli, okwenza kube ithuluzi eliwusizo lokufunda isakhiwo nokuhleleka kwe-DNA ngaphakathi kwamaseli. Iphinde isetshenziswe ku-flow cytometry ukuhlaziya okuqukethwe kwe-DNA kumaseli ngamanye nakwamanye amasu okubona nokulinganisa i-DNA. Ukusondelana okuphezulu kwe-DAPI ne-DNA kanye nezakhiwo zayo ezikhanyayo kuyenza ibe ithuluzi eliguquguqukayo nelisetshenziswa kabanzi ocwaningweni lwe-molecular and cell biology.