I-Coomassie brilliant blue R-250 CAS:6104-59-2 Isithombe Esivelele

I-Coomassie eluhlaza okwesibhakabhaka ekhazimulayo i-R-250 CAS:6104-59-2

I-Coomassie Brilliant Blue R-250 iyidayi evame ukusetshenziswa ocwaningweni lwamaprotheni kanye ne-electrophoresis. Iphawuleka kakhulu ngokusetshenziswa kwayo ekudayiseni amaprotheni ku-polyacrylamide gel electrophoresis (PAGE). Ngenxa yokusondela kwayo okunamandla kumaprotheni, iyabopha kuwo, okuholela kumbala oluhlaza okwesibhakabhaka obonakala kakhulu ovumela ukubonakala kanye nokulinganiswa kwamaprotheni. Ngaphezu kwalokho, ivame ukusetshenziswa ezinqubweni zokuhlanza amaprotheni njengodayi wokuhlola ukuhlushwa kwamaprotheni nokuhlola impumelelo yenqubo yokuhlanza.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

I-Coomassie Brilliant Blue R-250 iyidayi esetshenziswa kabanzi ku-biochemistry kanye ne-molecular biology. Ukusetshenziswa kwayo okuyinhloko kusekudayeni amaprotheni ku-polyacrylamide gel electrophoresis (PAGE). Uma ixutshwe nesampula yephrotheni bese ifakwa kujeli, idayi ye-Coomassie ibopha amaprotheni, iwavumele ukuthi abonakale njengamabhande aluhlaza okwesibhakabhaka ahlukile. Le ndlela yokufaka imibala ibalulekile ekuhlaziyeni nasekuchazeni amaprotheni ngokusekelwe esisindweni sawo sama-molecule kanye nobuningi obuhlobene kusampula. Ngaphezu kokusetshenziswa okusekelwe kujeli, i-Coomassie Brilliant Blue R-250 isetshenziswa nasezinqubweni zokuhlanza amaprotheni ukuhlola ukuhlushwa kwamaprotheni kanye nobumsulwa.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C45H46N3NaO7S2
Ukuhlolwa	99%
Ukubukeka	Impuphu ebomvu
Inombolo ye-CAS	6104-59-2
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.