I-Bis[2-Hydroxyethyl] imino Tris-(Hydroxymethyl)-methane CAS:6976-37-0

I-Agent Yokubhafa: I-Bicine isebenza njenge-ejenti yokuvimba futhi isiza ukugcina i-pH ezinzile ezixazululweni zamanzi. Inebanga elisebenzayo lokuvimba eliqala ku-pH 7.6 kuya ku-9.0, okwenza ibe usizo ekusetshenzisweni kwamakhemikhali kanye ne-biological.

Ukuhlolwa Kwe-Enzyme: I-Bicine ivame ukusetshenziswa ekuhlolweni kwe-enzyme kanye nasekuhlolweni kwamakhemikhali ngenxa yokuhambisana kwayo nokusabela kwe-enzyme. Isiza ukugcina i-pH efanele yomsebenzi we-enzyme, okuvumela ukulinganiswa okunembile kwe-kinetics ye-enzyme kanye nomsebenzi.

I-Cell Culture Media: I-Bicine isetshenziswa kwi-cell culture media njengomlawuli we-pH ukuze kulondolozwe izimo zokukhula ezifanele zezinhlobo ezahlukene zamaseli. Ihlinzeka ngendawo ezinzile yokukhula kwamaseli futhi iqinisekisa ukuthi i-pH ihlala ngaphakathi kobubanzi obufunwayo.

Ukuhlanzwa Kwamaprotheni: I-Bicine isetshenziswa ezinqubweni zokuhlanza amaprotheni, ikakhulukazi ngesikhathi se-chromatography yokushintshana kwama-ion. Isiza ekuhlukaniseni amaprotheni futhi inikeze amandla okugcina ukuqina kweprotheni ehlanziwe.

I-Electrophoresis: I-Bicine ivame ukusetshenziswa njenge-ejenti yokubopha kumasu e-gel electrophoresis, njenge-polyacrylamide gel electrophoresis (PAGE). Isiza ukugcina i-pH ezinzile ku-gel, okubalulekile ekuhlukaniseni nasekuhlaziyeni amaprotheni nama-nucleic acid.

Ukwakheka Kwemithi: I-Bicine isetshenziswa ekwakhiweni kwemikhiqizo yemithi ukulungisa nokuqinisa i-pH yesisombululo. Ingatholakala emithini eyahlukahlukene, okuhlanganisa ukwakheka koketshezi, imijovo, kanye nokulungiswa kwangaphandle.