I-Bis[2-Hydroxyethyl] imino Tris-(Hydroxymethyl)-methane CAS:6976-37-0

I-Buffering Agent: I-Bicine isebenza njenge-ejenti ephazamisayo futhi isiza ukugcina i-pH ezinzile ezixazululweni ezinamanzi.Inobubanzi obusebenzayo bokubhafa obungu-pH 7.6 ukuya ku-9.0, okuyenza isebenziseke ezinhlelweni zamakhemikhali ezinto eziphilayo kanye nezebhayoloji.

Ama-Enzyme Assays: I-Bicine ivamise ukusetshenziswa ekuhlolweni kwe-enzyme kanye nokuhlolwa kwe-biochemical ngenxa yokuhambisana kwayo nokusabela kwe-enzymatic.Kuyasiza ukugcina i-pH ephezulu yomsebenzi we-enzyme, okuvumela ukulinganiswa okunembile kwe-enzyme kinetics nomsebenzi.

I-Cell Culture Media: I-Bicine isetshenziswa kumidiya ye-cell culture njengesilawuli se-pH ukuze kugcinwe izimo zokukhula ezifanele zezinhlobo ezahlukene zamaseli.Ihlinzeka ngendawo ezinzile yokukhula kwamaseli futhi iqinisekisa ukuthi i-pH ihlala phakathi kwebanga elifiswayo.

Ukuhlanzwa Kwamaprotheni: I-Bicine isetshenziswa ezinqubweni zokuhlanza amaprotheni, ikakhulukazi phakathi ne-ion exchange chromatography.Isiza ekungeneni kwamaprotheni futhi inikeze amandla okuvimbela ukugcina ukuzinza kwephrotheni ehlanziwe.

I-Electrophoresis: I-Bicine ivamise ukusetshenziswa njenge-ejenti yokuvimbela kumasu e-gel electrophoresis, njenge-polyacrylamide gel electrophoresis (PAGE).Kuyasiza ukugcina i-pH ezinzile kujeli, okubalulekile ekuhlukaniseni nasekuhlaziyeni amaprotheni nama-nucleic acid.

Ukwakhiwa Kwemithi: I-Bicine isetshenziswa ekwakhiweni kwemikhiqizo yezemithi ukulungisa nokusimamisa i-pH yesisombululo.Ingatholakala emithini ehlukahlukene, okuhlanganisa ukwakheka kwe-liquid, imijovo, kanye namalungiselelo asendaweni.