I-ABTS (2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt) CAS:30931-67-0 Isithombe Esifakiwe

I-ABTS (2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) usawoti we-diammonium) CAS:30931-67-0

I-Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), evame ukubizwa ngokuthi i-ABTS, i-chromogenic substrate evame ukusetshenziswa ekuhlolweni kwamakhemikhali ezinto eziphilayo, ikakhulukazi emkhakheni we-enzymology.Kuyinhlanganisela yokwenziwa esetshenziselwa ukukala umsebenzi wama-enzyme ahlukahlukene, kufaka phakathi ama-peroxidase nama-oxidase.

I-ABTS ayinambala ngesimo sayo se-oxidized kodwa iphenduka ibe luhlaza okwesibhakabhaka-luhlaza lapho ifakwa i-enzyme lapho kukhona i-hydrogen peroxide noma umoya-mpilo wamangqamuzana.Lokhu kushintsha kombala kungenxa yokwakheka kwe-cation radical, edonsa ukukhanya ku-spectrum ebonakalayo.

Ukusabela phakathi kwe-ABTS kanye ne-enzyme kukhiqiza umkhiqizo onemibala ongakalwa nge-spectrophotometrically.Ukuqina kombala kuhambisana ngokuqondile nomsebenzi we-enzymatic, okuvumela abacwaningi ukuthi bahlole ngobuningi i-enzyme kinetics, inhibition ye-enzyme, noma ukusebenzisana kwe-enzyme-substrate.

I-ABTS inohlu olubanzi lwezinhlelo zokusebenza emikhakheni eyahlukene, okuhlanganisa ukuxilonga emitholampilo, ucwaningo lwezemithi, nesayensi yokudla.Izwela kakhulu futhi inikeza ububanzi obubanzi obuguquguqukayo, okuyenza ibe ukukhetha okudumile ezivivinyweni eziningi ze-biochemical.

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Ama-Enzymatic Assays: I-ABTS isetshenziswa kabanzi ukukala umsebenzi wama-enzyme afana nama-peroxidase nama-oxidase.Isebenza njenge-substrate yalawa ma-enzyme, futhi umsebenzi wawo ungalinganiswa ngokulinganisa ukuqina komkhiqizo onemibala owenziwe.

Ama-Antioxidant Capacity Assays: I-ABTS ivamise ukusetshenziswa ekuhloleni amandla okulwa ne-antioxidant ukuze kutholwe amandla ezinto okukhipha noma ukuvimbela ama-radicals mahhala.Ukwakheka kombala ebukhoneni be-antioxidant kukhombisa amandla ayo okukhipha amanzi.

Ama-protein Assays: I-ABTS ingasetshenziswa ukuhlola ingqikithi yamaprotheni kumasampuli ebhayoloji.Ukusabela kwe-ABTS ngethusi eliboshwe ngamaprotheni kuholela ekwakhekeni komkhiqizo onemibala ongalinganiswa.Le ndlela yaziwa kakhulu ngokuthi i-bicinchoninic acid (BCA) assay.

Ukutholwa Kwezidakamizwa: I-ABTS isetshenziswa ekuhlolweni kokuhlolwa okuphezulu ukuze kuhlolwe imisebenzi ye-antioxidant yezinhlanganisela zezidakamizwa ezingaba khona.Lokhu kuvumela abacwaningi ukuthi babone izinhlanganisela ezinemiphumela yokwelapha engaba khona.

Imboni Yokudla Neziphuzo: I-ABTS isetshenziswa embonini yokudla neziphuzo ukuze kuhlolwe amandla okulwa ne-antioxidant emikhiqizweni ehlukahlukene yokudla, njengezithelo, imifino, neziphuzo.Kuyasiza ukuhlola izinzuzo zezempilo ezingaba khona nokuzinza kwale mikhiqizo.

Ukuqapha Ezemvelo: I-ABTS ingaqashwa ukuze kuhlolwe umthamo we-antioxidant ophelele wamasampula emvelo, ukusiza ekuhlolweni kwamazinga okungcola kanye nomthelela wawo endaweni ezungezile.

Ukupakishwa komkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	I-C18H24N6O6S4
Isivivinyo	99%
Ukubukeka	Impushana eluhlaza
Inombolo ye-CAS.	30931-67-0
Ukupakisha	Ezincane nezinkulu
I-Shelf Life	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	ISO.