5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

I-5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) i-chromogenic substrate evame ukusetshenziselwa ukutholwa komsebenzi we-beta-glucuronidase (GUS).I-GUS iyi-enzyme etholakala ezintweni ezihlukahlukene, okuhlanganisa amagciwane, izitshalo nezilwane ezincelisayo.I-X-Gluc ivamise ukusetshenziswa ekuhlolweni kwezintatheli ze-GUS kanye nocwaningo lwebhayoloji yamangqamuzana.

Ukusetshenziswa okuyinhloko kwe-X-Gluc kusezindleleni ze-histochemical staining, lapho ikwazi ukubona ngeso lengqondo inkulumo nomsebenzi we-enzyme ye-GUS.Le substrate iyakwazi ukungena ngaphakathi kweseli futhi iba yi-hydrolyzed yi-GUS, okuholela ekwakhekeni kwemvula eluhlaza okwesibhakabhaka noma umkhiqizo ongancibiliki.Lokhu kungcolisa okuluhlaza okwesibhakabhaka kuvumela abacwaningi ukuthi bakhombe futhi benze okwasendaweni umsebenzi we-GUS kumaseli, izicubu, kanye nezinto zonke eziphilayo.

I-X-Gluc ingasetshenziswa futhi ekuhloleni kwenani ukukala umsebenzi we-GUS enzyme.Ukuqina kombala oluhlaza okwesibhakabhaka noma inani lomkhiqizo owenziwe lingahlotshaniswa nezinga lenkulumo ye-GUS noma umsebenzi wayo we-enzymatic.

Ukwengeza, i-X-Gluc iye yaqashwa ocwaningweni lwezakhi zofuzo ukuze kufundwe izakhi zofuzo, umsebenzi womgqugquzeli, nokuguqulwa kwezitshalo.Iphinde yasetshenziswa ezinhlelweni zebhaktheriya zokuhlanganisa kanye nokuthola amaphrotheni e-GUS fusion.