4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7

Ukuhlolwa komsebenzi we-enzyme: I-hydrolysis ye-4-Nitrophenyl-beta-D-glucopyranoside ngama-enzyme afana ne-β-glucosidase ingasetshenziswa ukukala umsebenzi we-enzyme.Ukukhululwa kwe-4-nitrophenol kungalinganiswa kusetshenziswa i-spectrophotometry, okuvumela ukunqunywa kwe-enzyme kinetics kanye nezifundo zokuvimbela.

Ukuchazwa kofuzo kanye nokuhlolwa kwezintatheli: I-4-Nitrophenyl-beta-D-glucopyranoside ingahlanganiswa namathegi athile wamangqamuzana ahlanganiswe kumaprotheni e-fusion.Umsebenzi wama-enzyme wephrotheni ehlanganisiwe ungabe usulinganiswa kalula ngokulinganisa ukukhishwa kwe-4-nitrophenol, okuhlinzeka ngemininingwane ekukhulumeni kofuzo kanye nokusebenzisana kwamaprotheni-namaprotheni.

Ukutholwa kwezidakamizwa kanye nokuhlolwa komphumela ophezulu: I-hydrolysis ye-4-Nitrophenyl-beta-D-glucopyranoside ingasetshenziswa njengokuhlola umsebenzi wokutholwa ukuze kutholwe izidakamizwa.Ivumela ukuhlolwa okusheshayo kwemitapo yolwazi eyinhlanganisela ukuze kuhlonzwe izithiyo noma izishoshovu zama-enzyme ezingaba khona.

Ukuhlolwa kokuxilonga: Ezinye izifo zihlotshaniswa noshintsho emisebenzini ethile yama-enzyme.Ngokusebenzisa i-hydrolysis ye-4-Nitrophenyl-beta-D-glucopyranoside ngama-enzyme athile njengokuhlola kokuxilonga, lezi zifo zingatholwa noma ziqashwe.Lokhu kufaka phakathi izimo ezihlobene nokuphazamiseka kokulondolozwa kwe-lysosomal kanye nezifo ze-hepatobiliary.