2,3,5-Triphenyl-2H-tetrazolium chloride CAS:298-96-4

I-2,3,5-Triphenyl-2H-tetrazolium chloride, eyaziwa nangokuthi i-TTC, iyinhlanganisela evame ukusetshenziswa njengesibonakaliso se-redox ekusetshenzisweni kwezinto eziphilayo kanye namakhemikhali. Ibonakala njengempuphu yekristalu ephuzi-mhlophe.

I-TTC ivame ukusetshenziswa ezivivinyweni ezahlukene zezinto eziphilayo kanye nokuhlolwa ukuze kunqunywe ukusebenza kahle kwamaseli kanye nomsebenzi we-metabolic. Uma ingezwa kumaseli aphilayo, incishiswa ngama-enzyme ukuze yakhe umkhiqizo we-red formazan. Lokhu kunciphisa kuncike kuma-enzyme e-dehydrogenase asebenzayo akhona kumaseli asebenzayo. Ngenxa yalokho, inani le-red formazan elikhiqizwayo lilingana nenani lamaseli asebenzayo ngokwe-metabolic.

Ikhono le-TTC lokuhlola ukuphila kweseli lenza libe usizo ezindaweni zocwaningo ezifana nokuhlolwa kwe-cytotoxicity, ukuhlolwa kokwanda kweseli, kanye nokuhlolwa kokusebenza kahle kwemithi. Ngaphezu kwalokho, isetshenziswa ku-microbiology ukuhlola ukukhula kwamagciwane, kanye nasezifundweni ezihilela ukukhuliswa kwezicubu zezitshalo kanye nokuhlolwa kokuphila kwezicubu.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukuhlolwa Kokuphila: I-TTC isetshenziswa kakhulu njengesibonakaliso sokuphila ezifundweni zezinto eziphilayo. Ivame ukusetshenziswa ukuhlola umsebenzi we-metabolic kanye nokuphila kwamaseli. Lapho i-TTC ingezwa kumaseli aphilayo, ama-enzyme e-dehydrogenase asebenzayo kumaseli asebenzayo ayayinciphisa ukuze yakhe umkhiqizo we-red formazan. Inani le-formazan elikhiqizwayo lilingana nenani lamaseli asebenzayo ngokwe-metabolic. Lokhu kuhlolwa kuyasiza ezindaweni ezahlukahlukene zocwaningo njengokuhlolwa kwe-cytotoxicity, ukuhlolwa kokusebenza kahle kwemithi, kanye nezifundo zokwanda kwamaseli.

Ukuhlolwa Kokukhula Kwamagciwane: I-TTC ingasetshenziswa ukuhlola ukukhula kwamagciwane kanye nokuphila kahle kwawo. Ukunciphisa i-TTC ngama-enzyme asebenzayo e-dehydrogenase kubonisa umsebenzi we-metabolic wamagciwane. Ivame ukusetshenziswa ukuhlola ukukhula kwamagciwane ekuhlolweni kwe-microbiology.

Ukuhlolwa Kokuphila Kwezicubu: I-TTC ithola ukusetshenziswa ekuhlolweni kokuphila kwezicubu ezinhlelweni ezahlukene zezinto eziphilayo, okuhlanganisa nokukhula kwezicubu zezitshalo. Ngokulinganisa ukwehla kwe-TTC ezicutshini, abacwaningi banganquma ukusebenza kanye nomsebenzi we-metabolic wezicubu.

Isikhombisi se-Redox: I-TTC isebenza njengesikhombisi se-redox ekusabeleni kwamakhemikhali. Iyancipha lapho kukhona ama-enzyme e-dehydrogenase asebenzayo, okuholela ekwakhekeni komkhiqizo we-red formazan. Le mpahla ye-TTC ingasetshenziswa ekusabeleni nasekufundweni okuhlukahlukene kwe-redox.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C19H15ClN4
Ukuhlolwa	99%
Ukubukeka	Impuphu ephuzi engabonakali
Inombolo ye-CAS	298-96-4
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.