3,3,5,5 -I-Tetramethylbenzidine dihydrochloride hydrate I-CAS:207738-08-7

I-3,3', 5,5'-Tetramethylbenzidine dihydrochloride (TMB) iyi-substrate esetshenziswa kabanzi ekuhlolweni kwe-immunosorbent okuxhunywe yi-enzyme (ELISAs) nakwezinye izivivinyo ze-biochemical. Uma i-TMB i-oxidized yi-enzyme horseradish peroxidase (HRP) lapho kukhona i-hydrogen peroxide, ikhiqiza umbala oluhlaza okwesibhakabhaka. Lokhu kushintsha kombala kusetshenziselwa ukuthola ukuthi kukhona ama-antibodies anelebula le-HRP noma amanye amaprotheni, okuvame ukukhombisa ukuthi kukhona i-antigen ethile kusampula. Umbala oluhlaza okwesibhakabhaka okhiqizwa ukusabela uvame ukulinganiswa kusetshenziswa i-spectrophotometer, evumela ukukalwa kokuqina kokushintsha kombala futhi inikeza izindlela zokuhlobanisa ukuqina kombala nenani le-molecule eqondiwe esampula. I-TMB inezinhlelo zokusebenza eziningi ekuhlolweni kokuxilonga, ocwaningweni, nasekuthuthukisweni kwemithi, ikakhulukazi ekutholeni ama-antibodies, ama-hormone, ama-cytokine, namanye ama-biomolecule. Ukuzwela kwayo, ukuzinza, kanye nokulula kokusetshenziswa kwayo kwenza kube ithuluzi eliwusizo ezindaweni ezahlukene zelebhu zokuthola nokulinganisa ama-molecule athile.