2,3,5-Triphenyltetrazolium chloride CAS:298-96-4

I-2,3,5-Triphenyltetrazolium chloride, evame ukubizwa ngokuthi i-TTC, ivame ukusetshenziswa njengesibonakaliso se-redox ekusetshenzisweni kwezinto eziphilayo kanye namakhemikhali. Enye yezindlela zayo eziyinhloko zokusebenzisa iwukuhlola ukusebenza kahle kweseli kanye nomsebenzi we-metabolic. Uma i-TTC ingezwa kumaseli noma ezicutshini eziphilayo, ingancishiswa ngama-enzyme e-dehydrogenase ku-mitochondria, okuholela ekwakhekeni komkhiqizo we-formazan onombala obomvu.

Lokhu kusabela kuvame ukusetshenziselwa ukuhlola ukuphefumula kwamaseli kanye nomsebenzi we-metabolic ku-microbiology, i-cell biology, kanye nezifundo ze-physiology yezitshalo. Ukuqina kombala obomvu owakhiwe kuhambisana nomsebenzi wama-enzyme e-dehydrogenase futhi kungalinganiswa ngobuningi ukuhlola ukusebenza kanye nomsebenzi we-metabolic wamaseli noma izicubu.

I-TTC isetshenziswa futhi njengesibonakaliso sokuba khona kwamaseli aphilayo amagciwane kanye nokuhlola ukuthambekela kwamagciwane kuma-antimicrobial agents. Ngaphezu kwalokho, inezinhlelo zokusebenza ekuhlolweni komsebenzi wamagciwane enhlabathini kanye nasezindaweni ezahlukene zocwaningo lapho ukulinganisa umsebenzi we-metabolic wamaseli kubalulekile.