3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate CAS:75621-03-3 Isithombe Esifakiwe

I-3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate I-CAS:75621-03-3

I-CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) iyinsipho yokuhlanzisisa esetshenziswa kakhulu ku-biochemistry kanye ne-molecular biology. Iyinsipho yokuhlanzisisa ye-zwitterionic, okusho ukuthi ineqembu elishaje kahle kanye nelishaje kabi.

I-CHAPS yaziwa ngekhono layo lokuncibilikisa nokuqinisa amaprotheni e-membrane, okwenza ibe usizo ezinhlotsheni ezahlukene zokusetshenziswa njengokukhipha amaprotheni, ukuhlanza, kanye nokuwachaza. Iphazamisa ukusebenzisana kwamaprotheni e-lipid, ivumela amaprotheni e-membrane ukuthi akhishwe esimweni sawo sendabuko.

Ngokungafani nezinye izihlanzi, i-CHAPS imnene futhi ayinciphisi amaprotheni amaningi, okwenza kube ukukhetha okuhle kakhulu kokugcina isakhiwo kanye nokusebenza kwamaprotheni ngesikhathi sokuhlolwa. Kungasiza futhi ekuvimbeleni ukuhlangana kwamaprotheni.

I-CHAPS ivame ukusetshenziswa ezindleleni ezifana ne-SDS-PAGE (i-sodium dodecyl sulfate polyacrylamide gel electrophoresis), i-isoelectric focusing, kanye ne-Western blotting. Iphinde isetshenziswe kaningi ezifundweni ezihilela ama-enzyme aboshwe yi-membrane, i-signal transduction, kanye nokusebenzisana kwamaprotheni nama-lipid.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukukhishwa Kwamaprotheni: I-CHAPS ivame ukusetshenziswa ukukhipha amaprotheni e-membrane kumasampula ebhayoloji. Iyasiza ekuncibilikiseni lawa maprotheni nokugcina isakhiwo sawo sendabuko.

Ukuhlanzwa Kwamaprotheni: I-CHAPS isetshenziswa ezindleleni ezahlukene zokuhlanza amaprotheni, njenge-affinity chromatography. Ingafakwa kuma-buffer okuhlanza ukuze incibilikise futhi izinzise amaprotheni e-membrane ngesikhathi senqubo yokuhlanza.

Ukuhlukaniswa Kwamaprotheni: I-CHAPS ivame ukusetshenziswa ezifundweni ezihilela ukuhlukaniswa kwamaprotheni e-membrane. Isiza ukugcina isakhiwo kanye nokusebenza kwamaprotheni ngesikhathi sezinqubo zokuhlola ezifana nokuhlolwa komsebenzi we-enzyme, ukusebenzisana kwamaprotheni namaprotheni, kanye nokuhlaziywa kwe-spectroscopic.

Izifundo Zamaprotheni E-Membrane: Amaprotheni e-Membrane adlala indima ebalulekile ezinqubweni eziningi zamaseli. I-CHAPS ivame ukusetshenziswa ocwaningweni oluhlobene nokudluliselwa kwesignali, umsebenzi wesiteshi se-ion, ukusebenzisana kwamaprotheni nama-lipid, kanye nokwakheka kwe-crystallization yamaprotheni e-membrane.

I-Electrophoresis: I-CHAPS isetshenziswa kumasu afana ne-SDS-PAGE kanye ne-isoelectric egxile ekuncibilikiseni amaprotheni e-membrane futhi kube lula ukuhlukaniswa kwawo kanye nokuhlaziywa kwawo.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C32H58N2O7S
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe ekristalu
Inombolo ye-CAS	75621-03-3
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.